Browsing by Author "Heinemann, Matthias"

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Carbon-13 labelling strategy for studying the ATP metabolism in individual yeast cells by micro-arrays for mass spectrometry
(2011)
Urban, Pawel L.
;
Schmidt, A. Mareike
;
Fagerer, Stephan R.
;
Amantonico, Andrea
;
Ibanez, Alfredo
Isotopic labelling of cellular metabolites, used in conjunction with high-density micro-arrays for mass spectrometry enables observation of ATP metabolism in single yeast cells.
Mass spectrometry-based metabolomics of single yeast cells
(2013-05-28)
Ibaneza, Alfredo J.
;
Fagerer, Stephan R.
;
Schmidt, Anna Mareike
;
Urban, Pawel L.
;
Jefimovs, Konstantins
Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (Delta PFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.