Browsing by Author "Hon, Kai-Wei"

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Synthesis of Oligomeric Mannosides and Their Structure-Binding Relationship with Concanavalin A
(2014-07-01)
Li, Chen-Wei
;
Hon, Kai-Wei
;
Ghosh, Bhaswati
;
Li, Po-Han
;
Lin, Hsien-Ya
Small glycodendrimers with a-mannosyl ligands were synthesized by using copper-catalyzed azide-alkyne coupling chemistry and some of these molecules were used as multivalent ligands to study the induction of concanavalin A (Con A) precipitation. The results showed that the monovalent mannose ligand could induce the precipitation of Con A. This unexpected finding initiated a series of studies to characterize the molecular basis of the ligand-lectin interaction. The atypical precipitation is found to be specific to the mannose, fluorescein moiety (FITC), and Con A. Apparently the mannose ligand binds to Con A through hydrogen-bonding interactions, whereas the binding of FITC is mediated by hydrophobic forces.