Browsing by Author "Hong, Wun-Yan"

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Conversion of absorption to fluorescence probe in solid-state sensor for nitric oxide and nitrite
(2013-04-01)
Hong, Wun-Yan
;
Yang, Tsung-Wei
;
Wang, Chun-Ming
;
Syu, Jia-Hao
;
Lin, Yu-Chao
A hydrogel bi-layer structure is used to convert an absorption type to a more convenient fluorescent type probe film for aqueous nitric oxide (NO) and nitrite (NO2-). In the bottom layer the commercial probe molecule 1,2-diaminoanthraquinone (DAQ) is dispersed in poly 2-hydroxyethyl methacrylate (poly HEMA), in the top layer the red inorganic phosphor is dispersed in poly HEMA. Both optical excitation and detection is from the bottom. This bi-layer is integrated with organic light-emitting diode (OLED) and organic photo-detector to form a real-time solid-state sensor. The sensor is responsive to NO bubbling, and remains stable in acid condition. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.
Electrospun Fibers as a Solid-State Real-Time Zinc Ion Sensor with High Sensitivity and Cell Medium Compatibility
(2013-03-25)
Syu, Jia-Hao
;
Cheng, Yi-Kai
;
Hong, Wun-Yan
;
Wang, Hsing-Ping
;
Lin, Yu-Chao
meso-2,6-Dichlorophenyltripyrrinone (TPN-Cl2), a probe molecule for zinc II ions, is dispersed in a polymer host. The red fluorescence peak at 620 nm appears when the molecule forms a complex with zinc at its center. TPN-Cl2 has a high selectivity for zinc II and tolerates many common metal ions present in the human body. The probe molecules are blended with a hydrogel polymer, poly(2-hydroxyethyl methacrylate) (poly HEMA), with 30 wt% dimethylformamide (DMF). The fiber structure with 1 m diameter is made by electrospinning in DMF solution of the probe and poly HEMA mixture. The fibrous film detects zinc ions with concentrations as low as 106 M in real-time both in water and in the commonly used cell culture liquid media Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS), which contain many metal ions and proteins. The time-resolution is 5 min for 106 M and 1 min for 105 M. This sensitivity and response speed satisfy the requirements for non-invasive biomedical studies.