Browsing by Author "Hsiao, Chun-Jen"

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A quadrupole ion trap mass spectrometer for dry microparticle analysis
(2019-09-21)
Ozdemir, Abdil
;
Lin, Jung-Lee
;
Gulfen, Mustafa
;
Hsiao, Chun-Jen
;
Chen, Chung-Hsuan
In this work, we report a new design of a charge detection quadrupole ion trap mass spectrometer (QIT-MS) for the analysis of micro-sized dry inorganic and bioparticles including red blood cells (RBCs) and different sizes of MCF-7 breast cancer cells. The developed method is one of the fastest methods to measure the mass of micro-sized particles. This system allows the online analysis of various micro-sized particles up to 1 x 10(17) Da. The calibration of the mass spectrometer has been done by using different sizes of polystyrene (PS) particles (2-15 mu m). The measured masses of RBCs were around 1.8 x 10(13) Da and MCF-7 cancer cells were between 1 x 10(14) and 4 x 10(14) Da. The calculated mass distribution profiles of the particles and cells were given as histogram profiles. The statistical data were summarized after Gaussian type fitting to the experimental histogram profiles. The new method gives very promising results for the analysis of particles and has very broad application.
ESI MS for Microsized Bioparticles
(2017-12-19)
Ozdemir, Abdil
;
Lin, Jung-Lee
;
Gulfen, Mustafa
;
Lai, Szu-Hsueh
;
Hsiao, Chun-Jen
An ESI ion trap mass spectrometer was designed for high-throughput and rapid mass analysis of large bioparticles. Mass calibration of the instrument was performed using commercially available polystyrene (PS) microparticles with a size comparable to cancer cells. Different sites of MCF-7 breast cancer cells (8 to 15 mu m) were used in this study. The masses of different cancer cells were measured. This system allows for the analysis of all types of particles.
Ultrasound-Enhanced Delivery of an Aptamer-Doxorubicin Conjugate to Breast Cancer Cells
(2019-01-01)
Chang, Ju-Yao
;
Ju, Tsai-Kai
;
Chen, Nelson G.
;
Hsiao, Chun-Jen
;
Wang, Chin-Yu
When treating cancer with chemotherapy, serious side effects are caused by the inability of the drug to be solely delivered to the tumor. As a result, a portion of the drug agents is inevitably delivered elsewhere and destroy normal cells. We report the first results on combining ultrasound with an aptamer-doxorubicin conjugate to treat cancer cells. Enhancement of therapeutic effects combined with a reduction in side effects indicates the potential of this approach. Although many studies have noted that ultrasound can enhance drug delivery, ultrasound has not addressed the goal of reducing side effects. To both reduce side effects and enhance the efficiency of killing cancer cells, this study mainly uses a specially targeted aptamer conjugated to the anticancer drug doxorubicin (DOX), which was applied in combination with ultrasound for cancer treatment. We also compared the results between cancer and normal cell lines to explore the targeting effect of the aptamer. Both breast cancer cells (MCF-7) and breast cells (MCF-10A) were used for the experiments. The results show that the aptamer conjugated to an anticancer drug can be used to target cancer cells and ultrasound can enhance drug delivery. This method can significantly reduce the side effects of the anticancer drug and achieve favorable therapeutic effects.