Browsing by Author "Hsieh, Yao-Yu"

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ABLGenomic Editing Sufficiently Abolishes Oncogenesis of Human Chronic Myeloid Leukemia Cells In Vitro and In Vivo
(2020-06-01)
Chen, Shu-Huey
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Hsieh, Yao-Yu
;
Tzeng, Huey-En
;
Lin, Chun-Yu
;
Hsu, Kai-Wen
Chronic myelogenous leukemia (CML) is the most common type of leukemia in adults, and more than 90% of CML patients harbor the abnormal Philadelphia chromosome (Ph) that encodes the BCR-ABL oncoprotein. Although the ABL kinase inhibitor (imatinib) has proven to be very effective in achieving high remission rates and improving prognosis, up to 33% of CML patients still cannot achieve an optimal response. Here, we used CRISPR/Cas9 to specifically target theBCR-ABLjunction region in K562 cells, resulting in the inhibition of cancer cell growth and oncogenesis. Due to the variety ofBCR-ABLjunctions in CML patients, we utilized gene editing of the humanABLgene for clinical applications. Using theABLgene-edited virus in K562 cells, we detected 41.2% indels inABLsgRNA_2-infected cells. TheABL-edited cells reveled significant suppression of BCR-ABL protein expression and downstream signals, inhibiting cell growth and increasing cell apoptosis. Next, we introduced theABLgene-edited virus into a systemic K562 leukemia xenograft mouse model, and bioluminescence imaging of the mice showed a significant reduction in the leukemia cell population inABL-targeted mice, compared to the scramble sgRNA virus-injected mice. In CML cells from clinical samples, infection with theABLgene-edited virus resulted in more than 30.9% indels and significant cancer cell death. Notably, no off-target effects or bone marrow cell suppression was found using theABLgene-edited virus, ensuring both user safety and treatment efficacy. This study demonstrated the critical role of theABLgene in maintaining CML cell survival and tumorigenicity in vitro and in vivo.ABLgene editing-based therapy might provide a potential strategy for imatinib-insensitive or resistant CML patients.
Compression and storage schemes in a sensor network with spatial and temporal coding techniques
(2008)
Wang, You-Chiun
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Hsieh, Yao-Yu
;
Tseng, Yu-Chee
;
資訊工程學系
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Department of Computer Science
Wireless sensor networks provide a convenient manner to monitor the physical environments. How to extend the network lifetime by reducing the amount of message transmissions is a critical issue. In this paper, we propose a multi-resolution compression and storage (MCS) framework to compress and preserve sensing data in a wireless sensor network. Our MCS framework adopts spatial and temporal compression schemes to reduce the amount of message transmissions, so the network lifetime can he prolonged and the network congestion can be alleviated. In addition, we also develop a storage mechanism to maintain sensing data in sensor nodes, so that users can query more detailed data when necessary. Our proposed methods consider the hardware limitations of sensor nodes. We also implement a prototyping system on the MICAz Mote platform.
HDAC1,2 Knock-Out and HDACi Induced Cell Apoptosis in Imatinib-Resistant K562 Cells
(2019-05-01)
Chen, Shu-Huey
;
Chow, Jyh-Ming
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Hsieh, Yao-Yu
;
Lin, Chun-Yu
;
Hsu, Kai-Wen
Since imatinib (Glivec or Gleevec) has been used to target the BCR-ABL fusion protein, chronic myeloid leukemia (CML) has become a manageable chronic disease with long-term survival. However, 15%-20% of CML patients ultimately develop resistance to imatinib and then progress to an accelerated phase and eventually to a blast crisis, limiting treatment options and resulting in a poor survival rate. Thus, we investigated whether histone deacetylase inhibitors (HDACis) could be used as a potential anticancer therapy for imatinib-resistant CML (IR-CML) patients. By applying a noninvasive apoptosis detection sensor (NIADS), we found that panobinostat significantly enhanced cell apoptosis in K562 cells. A further investigation showed that panobinostat induced apoptosis in both K562 and imatinib-resistant K562 (IR-K562) cells mainly via H3 and H4 histone acetylation, whereas panobinostat targeted cancer stem cells (CSCs) in IR-K562 cells. Using CRISPR/Cas9 genomic editing, we found that HDAC1 and HDAC2 knockout cells significantly induced cell apoptosis, indicating that the regulation of HDAC1 and HDAC2 is extremely important in maintaining K562 cell survival. All information in this study indicates that regulating HDAC activity provides therapeutic benefits against CML and IR-CML in the clinic.
iMouse: An integrated mobile surveillance and wireless sensor system
(2007-06-01)
Tseng, Yu-Chee
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Wang, You-Chiun
;
Cheng, Kai-Yang
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Hsieh, Yao-Yu
;
資訊工程學系
With their environment-sensing capability, wireless sensor networks can enrich human life in applications such as healthcare, building monitoring, and home security. The iMouse system integrates WSN technologies into surveillance technologies to support intelligent mobile surveillance services. The authors suggest several ways to improve or extend iMouse. One option is to facilitate mobile sensor navigation by, for example, integrating localization schemes to guide mobile sensors instead of using color tapes. A second option is to exploit coordination among mobile sensors, especially when they're on the road.
Multiresolution Spatial and Temporal Coding in a Wireless Sensor Network for Long-Term Monitoring Applications
(2009-06-01)
Wang, You-Chiun
;
Hsieh, Yao-Yu
;
Tseng, Yu-Chee
;
資訊工程學系
;
Department of Computer Science
In many WSN (wireless sensor network) applications, such as [1], [2], [3], the targets are to provide long-term monitoring of environments. In such applications, energy is a primary concern because sensor nodes have to regularly report data to the sink and need to continuously work for a very long time so that users may periodically request a rough overview of the monitored environment. On the other hand, users may occasionally query more in-depth data of certain areas to analyze abnormal events. These requirements motivate us to propose a multiresolution compression and query (MRCQ) framework to support in-network data compression and data storage in WSNs from both space and time domains. Our MRCQ framework can organize sensor nodes hierarchically and establish multiresolution summaries of sensing data inside the network, through spatial and temporal compressions. In the space domain, only lower resolution summaries are sent to the sink; the other higher resolution summaries are stored in the network and can be obtained via queries. In the time domain, historical data stored in sensor nodes exhibit a finer resolution for more recent data, and a coarser resolution for older data. Our methods consider the hardware limitations of sensor nodes. So, the result is expected to save sensors' energy significantly, and thus, can support long-term monitoring WSN applications. A prototyping system is developed to verify its feasibility. Simulation results also show the efficiency of MRCQ compared to existing work.