Browsing by Author "Hsieh, Yi-Ru"

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Functional Supramolecular Polymers: A Fluorescent Microfibrous Network in a Supramolecular Hydrogel for High-Contrast Live Cell-Material Imaging in 3D Environments
(2016-09-21)
Hsu, Shu-Min
;
Wu, Fang-Yi
;
Cheng, Hsun
;
Huang, Yu-Tang
;
Hsieh, Yi-Ru
A new bottom-up strategy based on aromatic peptide amphiphile is developed for a high-contrast visualization of 3D live cell-material imaging-something that has been difficult to achieve previously because of the problems associated with the diffraction of light by the nanosized peptide materials and the aggregation-caused quenching of aggregated pi-conjugated fluorophores in the nanostructures. This study reports an example of a novel supramolecular hydrogelator, naphthaleneimide-phenylalanine (NI-Phe), which forms a self-supporting hydrogel displaying a unique microfibrous network and promising aggregation-induced emission characteristics at pH 7.4. The storage modulus of the NI-Phe gel supports the mass of a cell for 3D cell culturing. This work illustrates a new dopant-free supramolecular approach, complementary to well-established doping procedures that should facilitate the development of live cell imaging in 3D scaffolding materials.
Reverse thermo-responsive hydrogels prepared from Pluronic F127 and gelatin composite materials
(2017-01-01)
Yeh, Mei-Yu
;
Zhao, Jiong-Yao
;
Hsieh, Yi-Ru
;
Lin, Jhong-Hua
;
Chen, Fang-Yi
Gelatin is an important hydrocolloid which has widespread applications in the pharmaceutical industry and biomedical science. However, despite its significance, clinical applications of gelatin are largely limited because of its gel-to-solution transition around human body temperature. In this work, we developed a series of F127-gelatin composite hydrogels with reverse thermo-responsive properties. The stiffness of F127-gelatin hydrogels can be adjusted by varying the types and contents of gelatin (type A, GA and type B, GB). The results of rheological data showed that F127-GA gels have higher gel strengths than those of F127-GB, which might be due to different isoelectric points between GA and GB. In addition, F127-gelatin gels showed significant cell viability and biocompatibility for human mesenchymal stem cells, indicating that these discovered hydrogels can serve as potential biomaterials and implantable scaffolds for applications in tissue engineering and regenerative medicine.