Browsing by Author "Hsiung, Chan-Te"

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Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor
(2018-01-21)
Tsai, Meng-Zhe
;
Hsiung, Chan-Te
;
Chen, Yang
;
Huang, Cheng-Sheng
;
Hsu, Hsin-Yun
We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU-1, which supports a limit of detection of 3.2 ng mL(-1) for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.
Refractive Index Sensor Based on a Gradient Grating Period Guided-Mode Resonance
(2019-02-01)
Hsiung, Chan-Te
;
Huang, Cheng-Sheng
;
機械工程學系
;
Department of Mechanical Engineering
In this letter, we introduce a novel refractive index sensor based on gradient grating period guided-mode resonance (GGP-GMR). Rather than a constant grating period, as in typical GMR, GGP-GMR comprises a grating period varying in increments of 2 nm. For grating variation from 386 to 392 nm, a detection range of 0.109 RIU with a bulk sensitivity of 1433.7 mu m/RIU and theoretical and experimental detection limits of 5.58 x 10(-3) and 10(-2) RIU, respectively, can be achieved. Several tactics that can be readily adopted are suggested to improve the limit of detection by an order of 10(-4) RIU, sufficient for detecting many clinically relevant biomarkers. The readout system requires a narrow band of light and a simple charge-coupled device combined with the compact design of the GGP-GMR sensor, which eases the miniaturization of the system for handheld devices as well as its integration with smartphones.