Browsing by Author "Hsu, Jen-Fang"

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Biothiol-triggered, self-disassembled silica nanobeads for intracellular drug delivery
(2015-09-01)
Huang, Xin-Chun
;
Wu, Li-Bang
;
Hsu, Jen-Fang
;
Shigeto, Shinsuke
;
Hsu, Hsin-Yun
Silica-based nanomaterials have demonstrated great potential in biomedical applications due to their chemical inertness. However, the degradability and endosomal trapping issues remain as rate-limiting barriers during their innovation. In this study, we provide a simple yet novel sol-gel approach to construct the redox-responsive silica nanobeads (ReSiNs), which could be rapidly disassembled upon redox gradient for intracellular drug delivery. The disulfide-linked scaffold of the nanobead was synthesized by employing the dithiobis-(succinimidyl propionate) to bridge (3-aminopropyI)-trimethoxysilane. Such silica matrix could be efficiently disrupted in response to intracellular glutathione, resulting in drug release and collapse of entire nanocarrier. Moreover, the ReSiNs exhibited insignificant cytotoxicity before and after the degradation. These results indicated the potential of using ReSiNs as a novel silica-based, biothiol-degradable nanoplatform for future drug delivery. (C) 2015 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.
When cells divide: Label-free multimodal spectral imaging for exploratory molecular investigation of living cells during cytokinesis
(2015-12-03)
Hsu, Jen-Fang
;
Hsieh, Pei-Ying
;
Hsu, Hsin-Yun
;
Shigeto, Shinsuke
;
應用化學系
In vivo, molecular-level investigation of cytokinesis, the climax of the cell cycle, not only deepens our understanding of how life continues, but it will also open up new possibilities of diagnosis/prognosis of cancer cells. Although fluorescence-based methods have been widely employed to address this challenge, they require a fluorophore to be designed for a specific known biomolecule and introduced into the cell. Here, we present a label-free spectral imaging approach based on multivariate curve resolution analysis of Raman hyperspectral data that enables exploratory untargeted studies of mammalian cell cytokinesis. We derived intrinsic vibrational spectra and intracellular distributions of major biomolecular components (lipids and proteins) in dividing and nondividing human colon cancer cells. In addition, we discovered an unusual autofluorescent lipid component that appears predominantly in the vicinity of the cleavage furrow during cytokinesis. This autofluorescence signal could be utilized as an endogenous probe for monitoring and visualizing cytokinesis in vivo.