Browsing by Author "Hsu, L."

Now showing 1 - 4 of 4

High Efficiency E-coli Concentration via Dynamic Light-induced Optoelectro-osmosis Flow on Organic Photoconductivity Biochip
(2011)
Yang, S. M.
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Lin, C. Y.
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Wang, T. P.
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Punde, T. H.
;
Chang, H. Y.
We demonstrate a dynamic light-induced optoelectro-osmosis flow design for high efficiency E. coli concentration on an organic photoconductive material based biochip. Several traditional approaches of bacteria purification and enrichment utilize magnetic beads coated with antibodies to attract them. The efficiency of collecting magnetic beads is equal to sort bacteria. Our work provides a direct method to enrich the E. coli concentration with dynamic light images. Due to the applications of photoconductive material, TiOPc, two features are demonstrated, simple biochip fabrication processes and rapid E. coli collection. The biochip is fabricated only with spin-coating a thin TiOPc layer, 500nm, on the indium tin oxide (ITO) conductive glass. Besides, the E. colt are concentrated together within 30 sec toward the illuminating region by the surface charge slip. We also calibrate the dominating effects in different applied AC frequency region. In conclusion, our light-induced E. coli manipulation approach would expand the scope of the examination efficiency, for example, water quality.
NUSAS: NEGATIVE PRESSURE DRIVING HEPG2/3T3 CELLS MIXING/GRADIENT CO-CULTURE INSIDE U TRAPPER ARRAY ON RAPID MULTICELLULAR SPHEROID ASSEMBLING SYSTEM
(2012)
Yang, S. M.
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Fu, C. Y.
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Tseng, S. Y.
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Srinivasu, V. P.
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Shilpa, S.
This article presents a Negative-pressure-powered U-trapper of Spheroid Assembling System (NUSAS) and four unique features are addressed: (1) Negative pressure powered medium driving, (2) HepG2/3T3 gradient distribution and mixing co-culture, (3) Cell trapping effect via gravity force and liquid flow co-activation, and (4) Large area of U trapper array. The development of in vitro liver device benefits liver toxicology and metabolism.[1,2] However, due to the complexity of human liver tissue, it has been rarely discussed what kind of hepatocyte to fibroblast ratio has nearest performance to a real liver tissue. Here, we culture liver/fibroblast cells with gradient ratio as multicellular assembling spheroids simultaneously to find this ratio. These results based on our efficient approaches give us meaningful foundations to investigate biomedical character of 3D HepG2/3T3 artificial tissue in different mixing cell conditions.
Using Infra-Red Laser and Nanodiamond Coating to Perform Spot Axotomy.
(2015)
Lin, Y.
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Huang, H.
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Chao, H.
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Hsu, L.
;
Hwang, E.
Using Infra-Red Laser and Nanodiamond Coating to Perform Spot Axotomy.
(2015)
Lin, Y.
;
Huang, H.
;
Chao, H.
;
Hsu, L.
;
Hwang, E.