Browsing by Author "Hsu, Shih-Lan"

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Effect of Anti-solvent Conditions on Low Density Supercritical Fluids Precipitation of Zeaxanthin Palmitates from Lycium Barbarum Fruits
(2014-03-01)
Lin, Kuo-Li
;
Chng, Lee-Muei
;
Lin, Justin Chun-Te
;
Hsu, Shih-Lan
;
Young, Chiu-Chung
A low density supercritical anti-solvent (SAS) precipitation of zeaxanthin palmitates (ZP) from Lycium barbarum (LB) to increase the purity of zeaxanthin palmitates in precipitates and to reduce the mean particle size of those precipitates was studied. The experimental data herein reveal that the purity of zeaxanthin palmitates increased with pressure, and total yield and purity increased with feed flow rate, but the effect of the anti-solvent time was trivial. A three-factor center composited schemed response surface method was used to study the effects of pressure, feed flow rate and time on total yield, purity, recovery and particle size of the precipitates. A precipitated powder with a mean particle size of approximately 2800 nm, a purity of 92.5%, a total yield of 66% and a recovery of 71%, was obtained at a pressure of 150 bar, a feed flow rate of 0.2 mL/min and an anti-solvent injection time of 48 min. This study demonstrated that low-density SAS precipitation promotes the purity of micro-sized ZP precipitates.
Effect of Anti-solvent Conditions on Low Density Supercritical Fluids Precipitation of Zeaxanthin Palmitates from Lycium Barbarum Fruits
(2014-03-01)
Lin, Kuo-Li
;
Chng, Lee-Muei
;
Lin, Justin Chun-Te
;
Hsu, Shih-Lan
;
Young, Chiu-Chung
A low density supercritical anti-solvent (SAS) precipitation of zeaxanthin palmitates (ZP) from Lycium barbarum (LB) to increase the purity of zeaxanthin palmitates in precipitates and to reduce the mean particle size of those precipitates was studied. The experimental data herein reveal that the purity of zeaxanthin palmitates increased with pressure, and total yield and purity increased with feed flow rate, but the effect of the anti-solvent time was trivial. A three-factor center composited schemed response surface method was used to study the effects of pressure, feed flow rate and time on total yield, purity, recovery and particle size of the precipitates. A precipitated powder with a mean particle size of approximately 2800 nm, a purity of 92.5%, a total yield of 66% and a recovery of 71%, was obtained at a pressure of 150 bar, a feed flow rate of 0.2 mL/min and an anti-solvent injection time of 48 min. This study demonstrated that low-density SAS precipitation promotes the purity of micro-sized ZP precipitates.
Genetic copy number variants in myocardial infarction patients with hyperlipidemia
(2011-11-30)
Shia, Wei-Chung
;
Ku, Tien-Hsiung
;
Tsao, Yu-Ming
;
Hsia, Chien-Hsun
;
Chang, Yung-Ming
Background: Cardiovascular disease is the chief cause of death in Taiwan and many countries, of which myocardial infarction (MI) is the most serious condition. Hyperlipidemia appears to be a significant cause of myocardial infarction, because it causes atherosclerosis directly. In recent years, copy number variation (CNV) has been analyzed in genomewide association studies of complex diseases. In this study, CNV was analyzed in blood samples and SNP arrays from 31 myocardial infarction patients with hyperlipidemia. Results: We identified seven CNV regions that were associated significantly with hyperlipidemia and myocardial infarction in our patients through multistage analysis (P<0.001), at 1p21.3, 1q31.2 (CDC73), 1q42.2 (DISC1), 3p21.31 (CDCP1), 10q11.21 (RET) 12p12.3 (PIK3C2G) and 16q23.3 (CDH13), respectively. In particular, the CNV region at 10q11.21 was examined by quantitative real-time PCR, the results of which were consistent with microarray findings. Conclusions: Our preliminary results constitute an alternative method of evaluating the relationship between CNV regions and cardiovascular disease. These susceptibility CNV regions may be used as biomarkers for early-stage diagnosis of hyperlipidemia and myocardial infarction, rendering them valuable for further research and discussion.
Low density supercritical fluids precipitation of 9-cis and all trans-beta-carotenes enriched particulates from Dunaliella salina
(2013-07-19)
Chen, Jian-Ren
;
Wu, Jia-Jiuan
;
Lin, Justin Chun-Te
;
Wang, Yuan-Chuen
;
Young, Chiu-Chung
In this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-beta-carotenes from Dunaliella saline. Total concentration of 9-cis (134.7 mg/g) and trans-beta-carotene (204.2 mg/g) was increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1 mg/g (355.6 for 9-cis and 576.5 for trans) of total beta-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total beta-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-beta-carotenes by low-density SAS was proved to be feasible and environmental benign. (C) 2013 Elsevier B.V. All rights reserved.
Low density supercritical fluids precipitation of 9-cis and all trans-beta-carotenes enriched particulates from Dunaliella salina
(2013-07-19)
Chen, Jian-Ren
;
Wu, Jia-Jiuan
;
Lin, Justin Chun-Te
;
Wang, Yuan-Chuen
;
Young, Chiu-Chung
In this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-beta-carotenes from Dunaliella saline. Total concentration of 9-cis (134.7 mg/g) and trans-beta-carotene (204.2 mg/g) was increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1 mg/g (355.6 for 9-cis and 576.5 for trans) of total beta-carotenes with a recovery of 86.3% (83.9% for cis and 87.8% for trans). Effects of two SAS operational conditions on the purity, recovery of total beta-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-beta-carotenes by low-density SAS was proved to be feasible and environmental benign. (C) 2013 Elsevier B.V. All rights reserved.