Browsing by Author "Huang, Chen-Han"

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Biocompatible Inhibitor Based on Chitosan and Amphiphilic Peptide against Mutant Huntingtin Toxicity
(1970-01-01)
Wahyuningtyas, Devi
;
Chen, Wen-Hao
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Huang, Chen-Han
;
He, Yu-Jung
;
Huang, Joseph Jen-Tse
Huntington's disease (HD) is classified as a protein-misfolding disease correlated with the mutant Huntingtin (mHtt) protein with abnormally expanded polyglutamine (polyQ) domains. Because no effective drugs have yet been reported, attempts to develop better therapy to delay the age of onset are in urgent demand. In this study, an amphiphilic peptide consisting of negatively charged hexaglutamic acid and a stretch of decaglutamine (E(6)Q(10)) was chemically synthesized as an inhibitor against polyQ and mHtt toxicity. It is found that E(6)Q(10) selfassembles into spherical vesicles, as shown by means of TEM, cryoelectron microscopy, and dynamic light scattering. Assembled E(6)Q(10) prevented the polyQ-rich peptide (KKWQ(20)AKK) from forming amyloid fibrils. To enable the cell-penetration ability of E(6)Q(10), the E(6)Q(10).chitosan complex was generated. It is demonstrated that the complex penetrates cells, interferes with the mHtt oligomerization and aggregation process, and prevents mHtt cytotoxicity. By combining positively charged chitosan and amphiphilic peptides with a negatively charge moiety, a new strategy is provided to develop biocompatible and biodegradable inhibitors against mHtt toxicity.
Surface-enhanced Raman scattering of graphene with photo-assisted-synthesized gold nanoparticles
(2013-03-11)
Cheng, Cheng-En
;
Lin, Chi-Yuan
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Chang, Hao-Yu
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Huang, Chen-Han
;
Lin, Hsing-Ying
This paper presents a convenient and reliable method to prepare gold nanoparticles (AuNPs) on graphene. Photo-assisted synthesis (PAS) was employed to grow AuNPs in AuCl4- electrolyte on graphene. The size of AuNPs could be as large as 130 nm. This optical method had a steady growth rate of AuNPs. The distribution of AuNPs was well controlled by focusing the laser for PAS. The minimum diameter of the distribution was approximately 1 mu m. Surface-enhanced Raman scattering of graphene due to AuNPs was observed. Electrical fields near AuNPs calculated by the finite-difference time-domain algorithm ensured that the Raman enhancement was attributed to the localized surface plasmons of AuNPs. (c) 2013 Optical Society of America