Browsing by Author "Huang, Chi-Chieh"

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Real-time Detection of alpha-Thrombin Binding to Single-Strand DNA Aptamers by a Highly Sensitive Si-Based Waveguide SPR Biosensor
(2012)
Huang, Chi-Chieh
;
Hsu, Hsin-Feng
;
Chen, Sz-Hau
;
Tsai, Kun-Yu
;
Huang, Yang-Tung
In this paper, real-time characterization of alpha-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with alpha-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for alpha-thrombin detection was achieved.