Browsing by Author "Huang, Hang-Ping"

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A novel optoelectrofulidic system for cells/particles manipulation and sorting
(2010)
Yang, Shih-Mo
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Yu, Tung-Ming
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Huang, Hang-Ping
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Hsu, Long
;
Liu, Cheng-Hsien
A novel optoelectrofulidic system integrated optical image concentration and alignment system, dielectrophoresis phenomenon, microfluidic and friendly real-time control interface is first reported in this article. A new application of photoconductive material oxotitanium phthalocyanine (TiOPc) for microparticle applying has been first described and demonstrated by our research group. Basis on the special character of the photoconductive material, a TiOPc-based optoelectronic tweezers (Ti-OET) is utilized for single and massive cells/particles manipulation. The objects wanted to be manipulated are defined with different behaviors (e.g., press, release, drag and move) using Flash (R) software when the cursor acts on them. It also reveals the application for biological application to form the cells trapping with three sorts of cells, HMEC-1, HepG2 and HEK293t. Another application of our optoelectrofulidic system is to fabricate a TiOPc-based flow cytometry chip which can be used for sorting the 15 mu m diameter particles with 105 mu m/s velocity. When the 10Vp. p. voltage and 45 kHz AC frequency apply on the top and button ITO electrode, the illuminated light pattern will become a spatially virtual switch inside the microchannel. The dielectrophoresis force between top ITO glass and button photoconductive layer controlled by the friendly interface will concentrate the cells/particles as a straight line and individually direct each one in different paths. In summary, we have established an optoelectronfulidic-based chip and spatially virtual switch system which are applied for cell pattern and particles sorting. In the future, this easy manipulation approach can place the full power of optoelectronfulidic chip into the biological operators' hands.
An easier way to improvement the trapping performance of optical tweezers by donut-shaped beam
(2010)
Tsai, Che-Liang
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Huang, Hang-Ping
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Hsu, Long
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Hsu, Ken-Yuh
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光電工程學系
In optical tweezers, it can be comprehended that the larger the inclination angle between the condensed laser beam and the optical axis contributes more to axial trapping force, while the central part of laser beam with smaller inclination angle contributes more transverse trapping force. Therefore donut-shaped beam is used to improve the problem of less-axial trapping for common optical tweezers. Some research reports have shown that the efficiency of a trapping force can be enhanced by using a donut-shaped beam. In this paper we present the dependence of the axial and the transverse components of a trapping force on the configuration of a focused donut-shaped beam. The simulation result will provide a simple and easy guide for optical tweezers users to adjust the configuration of a focused donut-shaped beam for optimal trapping performance.
Dynamic manipulation and patterning of microparticles and cells by using TiOPc-based optoelectronic dielectrophoresis
(2010-06-15)
Yang, Shih-Mo
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Yu, Tung-Ming
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Huang, Hang-Ping
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Ku, Meng-Yen
;
Hsu, Long
We develop light-driven optoelectronic tweezers based on the organic photoconductive material titanium oxide phthalocyanine. These tweezers function based on negative dielectrophoresis (nDEP). The dynamic manipulation of a single microparticle and cell patterning are demonstrated by using this light-driven optoelectronic DEP chip. The adaptive light patterns that drive the optoelectronic DEP onchip are designed by using Flash software to approach appropriate dynamic manipulation. This is also the first reported demonstration, to the best of our knowledge, for successfully patterning such delicate cells from human hepatocellular liver carcinoma cell line HepG2 by using any optoelectronic tweezers. (C) 2010 Optical Society of America
Light-driven manipulation of picobubbles on a titanium oxide phthalocyanine-based optoelectronic chip
(2011-04-11)
Yang, Shih-Mo
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Yu, Tung-Ming
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Huang, Hang-Ping
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Ku, Meng-Yen
;
Tseng, Sheng-Yang
Microbubbles have a variety of applications in science and biological technology. Here, we demonstrate the manipulation of the picoliter gas bubble (picobubble) based on the optoelectronic-mechanism. The organic photoconductive material, titanium oxide phthalocyanine (TiOPc), was developed to make the light-sensitive substrate of this optoelectronic chip. The virtual electrodes are formed by projecting the dynamic light pattern onto TiOPc layer for generating the desired nonuniform electric field. The picobubble suspended in silicone oil can be manipulated with the velocity of 40-50 mu m/s. The driving force up to 160 pico-Newtons could be generated for manipulating a gas bubble of 300 picoliters. (C) 2011 American Institute of Physics. [doi:10.1063/1.3580760]
ORGANIC PHOTOCONDUCTIVE DIELECTROPHORESIS BY USING TITANIUM OXIDE PHTHALOCYANINE FOR MICRO-PARTICLES MANUPULATION
(2010)
Yu, Tung-Ming
;
Yang, Shi-Mo
;
Huang, Hang-Ping
;
Ku, Meng-Yen
;
Tseng, Sheng-Yang
This paper reports our updating development for the organic photoconductive dielectrophoresis (OPDEP) by using titanium oxide phthalocyanine (TiOPc) material for the manipulation of micro-objects. The advantages of organic photoconductive material include non-toxicity, low cost, easy coating fabrication, flexibility, panchromaticity and high photosensitivity [1]. TiOPc has the high photoconductivity and the optical absorptions in the near infrared region (550 - 850 nm) to be a candidate of the light-sensitive charge generation layer. OPDEP chip is easily mass-produced by using a spin-coating process. Here, we demonstrate two kinds of the manipulations of micro-particles on our OPDEP chip. Firstly, the trapping and movement of single micro-particle is achieved by the ring shaped virtual electrode. Secondly, the steel rim shaped virtual electrode is designed to rotate micro-particles and characterize the OPDEP induced force.