Browsing by Author "Huang, Hsin-Yang"

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A Multitheragnostic Nanobubble System to Induce Blood-Brain Barrier Disruption with Magnetically Guided Focused Ultrasound
(2015-01-27)
Huang, Hsin-Yang
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Liu, Hao-Li
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Hsu, Po-Hung
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Chiang, Chih-Sheng
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Tsai, Chih-Hung
Magnetic-sensitive silica nanospheres for controlled drug release
(2008-01-01)
Hu, Shang-Hsiu
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Liu, Ting-Yu
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Huang, Hsin-Yang
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Liu, Dean-Mo
;
Chen, San-Yuan
Recently, magnetic silica-based nanospheres have received great attention and displayed magnificent potential for bioimaging and therapeutic purposes. This study provided a way to accelerate drug release from magnetic-sensitive silica nanospheres by controlled bursting to a therapeutically effective concentration by a high-frequency magnetic field (HFMF). The magnetic-sensitive silica nanospheres were synthesized by an in situ process, with particle sizes about 50 nm and able to release specific amounts of drug in a burst manner via short exposure to a HFMF. The HFMF accelerates the rotation of magnetic nanoparticles deposited in the silica matrix with generated heat energy and subsequently enlarges the nanostructure of the silica matrix to produce porous channels that cause the drug to be released easily. By taking these magnetic-responsive controllable drug release behaviors, the magnetic silica nanospheres can be designed for controlled burst release of therapeutic agents for especially urgent physiological needs.
Self-assembling PVA-F127 thermosensitive nanocarriers with highly sensitive magnetically-triggered drug release for epilepsy therapy in vivo
(2012)
Huang, Hsin-Yang
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Hu, Shang-Hsiu
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Chian, Chih-Shang
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Chen, San-Yuan
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Lai, Hsin-Yi
Self-assembling, crosslinker-free, highly thermosensitive nanocarriers (TSNCs) were synthesized by the incorporation of iron oxide nanoparticles and hydrophobic drug molecules into a thermosensitive matrix composed of PEO-PPO-PEO (F127) triblock-copolymer and polyvinyl alcohol (PVA) using a mini-emulsion process. The addition of PVA significantly contributes to the stability of the thermosensitive PVA-F127 nanocarriers and reduces drug leakage because it provides hydrogen bonds to react with the PEO segments in the shell. The TSNCs exhibit a remarkable triggered size contraction and shrinkage as a result of opposing polymer thermal effects. These effects include thermal expansion of the PVA and thermal shrinkage of the F127 when the magnetic field induced a temperature change that reached 40 to 50 degrees C through heat generation of the magnetic nanoparticles. Depending on the PVA/F127 ratios, the TSNCs can act as a remotely triggered drug delivery platform with a tunable burst drug release profile through the structure deformation by an external magnetic field. Furthermore, the TSNCs also presented ultrasensitive magnetic resonance imaging (MRI), as demonstrated by a relatively high r(2)/r(1) ratio (430). A preliminary in vivo study using the Long-Evans rat model has demonstrated a significant reduction in the spike-wave discharge after the anti-epilepsy drug, ethosuximide (ETX), was burst released from the TSNCs. These results were compared to PVA nanocarriers under the same magnetic induction as the in vitro carriers. Using a well-controlled burst release, these nanocarriers may provide significant advantages as highly temperature-responsive nanocarriers for the treatment of acute diseases.
SPIO nanoparticle-stabilized PAA-F127 thermosensitive nanobubbles with MR/US dual-modality imaging and HIFU-triggered drug release for magnetically guided in vivo tumor therapy
(2013-11-28)
Huang, Hsin-Yang
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Hu, Shang-Hsiu
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Hung, Shih-Ya
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Chiang, Chih-Sheng
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Liu, Hao-Li
Nanobubbles can serve as promising, next-generation theranostic platforms for ultrasound (US) and magnetic resonance (MR) imaging, and combined magnetic targeting (MT) and high-intensity focused ultrasound (HIFU)-triggered drug release for tumor therapy. Nanobubble-based dual contrast enhancement agents encapsulated with perfluoropentane and stabilized with superparamagnetic iron oxide (SPIO) nanoparticles have been synthesized through a single-step emulsion process from thermosensitive F127 and polyacrylic acid (PAA). Both US and MR imaging contrast can be optimized by varying the shell thickness and SPIO-embedded concentration. The US contrast can be enhanced from a mean gray value of 62 to 115, and the MR r(2) value can be enhanced from 164 to 208 (s(-1) mM(-1) Fe) by increasing the SPIO concentration from 14.1 to 28.2 mg/mL, respectively. In vivo investigations of SPIO-embedded nanobubbles in excised tumors under external MT revealed that the US and MR signals change quantitatively compared to the same site without MT. This combined strategy enables the nanobubbles to enhance both passive targeting (increasing the permeability by HIFU) and physical MT of chemotherapeutic drugs to tumors. The integration of functionalities makes this nanobubble system a powerful and viable new tool to achieve simultaneous in vivo tumor imaging and efficacious cancer therapy. (C) 2013 Published by Elsevier B.V.
Stimuli-Responsive Controlled Drug Release from Magnetic-Sensitive Silica Nanospheres
(2009-02-01)
Hu, Shang-Hsiu
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Liu, Ting-Yu
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Huang, Hsin-Yang
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Liu, Dean-Mo
;
Chen, San-Yuan
A novel method for control burst releasing of drug via a high frequency magnetic field (HFMF) from magnetic-sensitive silica nanospheres was developed. The nanospheres were synthesized by a combination of emulsion and sol-gel process with the particles controlled at about 80 nm in diameter. Under repeated exposures to the high frequency magnetic stimulus, the drug release behaviors showed reproducible slow-to-burst profiles while consecutively applying the magnetic stimulus at 10-min switching time and the release profile restored immediately when the stimulus was removed. By taking this non-contact control-burst method, the magnetic silica nanospheres can be designed to treat the cancer therapy and urgent physiological needs.