Browsing by Author "Huang, Hsuan-Yu"

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Fast incorporation of primary amine group into polylactide surface for improving C2C12 cell proliferation using nitrogen-based atmospheric-pressure plasma jets
(2014-01-01)
Yang, Yi-Wei
;
Wu, Jane-Yii
;
Liu, Chih-Tung
;
Liao, Guo-Chun
;
Huang, Hsuan-Yu
In this article, we report the development of the fast incorporation of primary amine functional groups into a polylactide (PLA) surface using the post-discharge jet region of an atmospheric-pressure nitrogen-based dielectric barrier discharge (DBD). Plasma treatments were carried out in two sequential steps: (1) nitrogen with 0.1% oxygen addition, and (2) nitrogen with 5% ammonia addition. The analyses show that the concentration of N/C ratio, surface energy, contact angle, and surface roughness of the treated PLA surface can reach 19.1%, 70.5 mJ/m(2), 38 degrees and 73.22 nm, respectively. In addition, the proposed two-step plasma treatment procedure can produce a PLA surface exhibiting almost the same C2C12 cell attachment and proliferation performance as that of the conventional gelatin coating method. Most importantly, the processing/preparation time is reduced from 13-15 h (gelatin coating method) to 5-15 min (two-step plasma treatment), which is very useful in practical applications. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 160-169, 2014.
Parallel synthesis and biological evolution of quinic acid derivatives as immuno-suppressing agents against T-cell receptors
(2015-01-01)
Huang, Chih-Yu
;
Chen, Li-Hsun
;
Huang, Hsuan-Yu
;
Kao, Feng-Sheng
;
Lee, Yun-Ta
A simple protocol for the synthesis of quinic acid derivatives was established and their biological evolution against T-cells is studied. Results showed that one of the derivatives, Cyn-1324, has low toxicity on T-cells and a high effect on reducing Signal 2 of T-cell immune responses. In vitro binding measurements of atomic force spectroscopy further indicated that the blocking effect of Cyn-1324 between CD28 and CD80 was about 31 + 4%. In vivo animal tests also confirmed that Cyn-1324 can reduce the allergic responses from ovalbumin-induced mice with little toxicity. Based on these observations, Cyn-1324 can be a mild immuno-suppressive candidate for future drug development.