Browsing by Author "Huang, Wei-Chieh"

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Functional gold nanoparticles as photothermal agents for selective-killing of pathogenic bacteria
(2007-12-01)
Huang, Wei-Chieh
;
Tsai, Pei-Jane
;
Chen, Yu-Chie
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應用數學系
;
應用化學系分子科學碩博班
Aims: Our aim was to demonstrate that functional gold nanoparticles can be used as photothermal agents for the selective killing of pathogenic bacteria. Materials & methods: Gold nanoparticles with polygonal shapes, capable of absorbing near infrared (NIR) light, were generated through a photochemical reaction. Vancomycin, which can bind with the terminal D-Ala-D-Ala moieties of the peptide units of pathogen cell walls, was immobilized on the surface of the gold nanoparticles. The vancomycin-bound gold nanoparticles were used as the photothermal agents for the inhibition of pathogenic bacteria growth, under irradiation of NIR light (808 nm). Results & discussion: We have demonstrated that vancomycin-bound gold nanoparticles are capable of selective-binding onto the cell walls of pathogenic bacteria. A large portion (> 99%) of bacteria targeted by the gold nanoparticles was destroyed under illumination by NIR light within 5 min owing to suffering from heating. Conclusions: This photothermal approach is effective for the inhibition of pathogenic bacteria cell growth, including Gram-positive bacteria, Gram-negative bacteria and antibiotic-resistant bacteria.
Functional nanoparticle-based antibiotics and preparation method thereof
(2010-11-04)
Chen, Yu-Chie
;
Huang, Wei-Chieh
The present invention is related to a novel functional nanoparticle-based antibiotics and preparation method thereof, especially related to an antibiotics-modified nanoparticle. The functional nanoparticle-based antibiotics according to the present invention can be used as the affinity probes and the photothermal agents to effectively inhibit the cell growth of pathogenic bacteria under NIR irradiation.
Multifunctional Fe(3)O(4)@Au Nanoeggs as Photothermal Agents for Selective Killing of Nosocomial and Antibiotic-Resistant Bacteria
(2009-01-01)
Huang, Wei-Chieh
;
Tsai, Pei-Jane
;
Chen, Yu-Chie
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應用化學系
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Department of Applied Chemistry
Multifunctional Fe3O4@Au Nanoeggs as Photothermal Agents for Selective Killing of Nosocomial and Antibiotic-Resistant Bacteria
(2009-01-01)
Huang, Wei-Chieh
;
Tsai, Pei-Jane
;
Chen, Yu-Chie
;
應用化學系
;
Department of Applied Chemistry
Photochemical synthesis of polygonal gold nanoparticles
(2008-04-01)
Huang, Wei-Chieh
;
Chen, Yu-Chie
;
應用化學系
;
應用化學系分子科學碩博班
;
Department of Applied Chemistry
In this paper, we propose a method to generate gold nanoparticles capable of absorbing near infrared light (NIR) radiation through a photochemical reaction. This approach does not require the use of either surfactants or polymers, reducing the difficulties that may arise in further chemical modifications for the gold nanoparticles. The gold nanoparticles with either triangular or hexagonal shapes were generated using the photo-reduction method, mixing hydrogen tetrachloroaurate with sodium oxalate, a reducing agent, in aqueous solution under illumination of a mercury lamp (lambda(max) = 306 nm) for more than 10 min. The size of the gold nanoparticles varies from 25 to 200 nm, which mainly depends on the duration of light illumination and the concentration of sodium oxalate. Furthermore, we demonstrate that the presence of the gold nanoparticles in aqueous solutions can effectively elevate the temperature of the solutions under irradiation of NIR light (808 nm) within a few minutes. The gold nanoparticles can be potentially used as suitable photothermal agents for hyperthermia.