Browsing by Author "Huang, Yu-Tzu"

Now showing 1 - 9 of 9

A comparison of two 16S rRNA gene-based PCR primer sets in unraveling anammox bacteria from different environmental samples
(2013-12-01)
Han, Ping
;
Huang, Yu-Tzu
;
Lin, Jih-Gaw
;
Gu, Ji-Dong
;
環境工程研究所
Two 16S rRNA gene-based PCR primer sets (Brod541F/Amx820R and A438f/A684r) for detecting anammox bacteria were compared using sediments from Mai Po wetlands (MP), the South China Sea (SCS), a freshwater reservoir (R2), and sludge granules from a wastewater treatment plant (A2). By comparing their ability in profiling anammox bacteria, the recovered diversity, community structure, and abundance of anammox bacteria among all these diverse samples indicated that A438f/A684r performed better than Brod541F/Amx820R in retrieving anammox bacteria from these different environmental samples. Five Scalindua subclusters (zhenghei-I, SCS-I, SCS-III, arabica, and brodae) dominated in SCS whereas two Scalindua subclusters (zhenghei-II and wagneri) and one cluster of Kuenenia dominated in MP. R2 showed a higher diversity of anammox bacteria with two new retrieved clusters (R2-New-1 and R2-New-2), which deserves further detailed study. The dominance of Brocadia in sample A2 was supported by both of the primer sets used. Results collectively indicate strongly niche-specific community structures of anammox bacteria in different environments, and A438f/A684r is highly recommended for screening anammox bacteria from various environments when dealing with a collection of samples with diverse physiochemical characteristics.
Insights into the roles of anammox bacteria in post-treatment of anaerobically-treated sewage
(2018-01-01)
Wang, Qian
;
Tan, Giin-Yu Amy
;
Azari, Mohammad
;
Huang, Xiaowu
;
Denecke, Martin
Complete anaerobic treatment of sewage has the potential to achieve net energy production via capturing the energy of organics in sewage, but not nitrogen, which remains a concern. Anaerobic ammonium oxidation (anammox) is potential for energy-efficient nitrogen removal from anaerobically-treated sewage. Given the sensitivity of anammox bacteria and the challenge of the low ammonium-bearing influent with reduced compounds such as volatile fatty acids, methane, and sulfide, this article critically reviewed and analyzed, using thermodynamic computation as a tool, the potential interactions of anammox bacteria with other microbes induced by the reduced compounds in anaerobically-treated sewage. The dynamics of anammox bacteria to cooperate with these microbes that metabolize non-ammonium pollutants and to outcompete for the common substrate, i.e., nitrite, are extensively discussed. The versatile metabolism of anammox bacteria on volatile fatty acids and iron, which would help facilitate the treatment efficiency, was also reviewed. The information offered in this review will be beneficial to researchers and engineers in controlling process stability, enhancing total nitrogen removal, and achieving better effluent quality in post-treatment of anaerobically-treated sewage by anammox-based processes.
Nanoarchitectured structure and population dynamics of anaerobic ammonium oxidizing (anammox) bacteria in a wastewater treatment plant
(2020-09-05)
Huang, Yu-Tzu
;
Chen, Shiou-Shiou
;
Jetten, Mike S. M.
;
Lin, Jih-Gaw
;
環境工程研究所
Studies on microbial community and population dynamics are essential for the successful development, monitoring and operation of biological wastewater treatment systems. Especially for novel or sustainable systems such as the anaerobic ammonium oxidizing (anammox) process that are not yet well explored. Here we collected granular microbial sludge samples and investigated a community of anammox bacteria over a period of four years, divided into eight stages in a full scale simultaneous partial nitrification, anammox and denitrification (SNAD) process for treating landfill leachate. Specific qPCR primers were designed to target and quantify the two most abundant anammox species, Candidatus Kuenenia stuttgartiensis (KS) and Candidatus Brocadia anammoxidans (BA). The two species were monitored and could explain the dynamic shift of the anammox community corresponding to the operating conditions. Using the newly designed KS-specific primer (KSqF3/KSqR3) and BA-specific primer (BAqF/BAqR), we estimated the amounts of KS and BA to be in the range of 6.2 x 10(6) to 5.9 x 10(8) and 1.1 x 10(5) to 4.1 x 10(7) copies mu g(-1) DNA, respectively. KS was found to be the dominant species in all anammox granules studied and played an important role in the formation of granules. The KS/BA ratio was positively correlated to the size of granules in the reactor and ammonia nitrogen removal efficiency of the treatment plant.
Nitrogen removal from opto-electronic wastewater using the simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) process in sequencing batch reactor
(2012-06-01)
Daverey, Achlesh
;
Su, Sin-Han
;
Huang, Yu-Tzu
;
Lin, Jih-Gaw
;
環境工程研究所
Simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) system was applied to treat the opto-electronic industrial wastewater in a 2.5 L sequencing batch reactor. The characteristics of wastewater were low C/N ratio (similar to 0.2) with 100 mg-COD/L and 567 mgNH(4)(+)-N/L. The experiment was carried out over 8 months in six different stages, where nitrogen loading rate was gradually increased from 16 g-N/m(3) d in stage I to 230 g-N/m(3) d in stage VI. The COD and nitrogen removal rates by the SNAD system reached to 28 g COD/m(3) d and 197 g NH4+-N/m(3) d, respectively in stage VI. These results showed that the SNAD system is suitable to treat wastewater containing high nitrogen pollutants with low COD level. Presence of ammonium oxidizing bacteria and anammox bacteria were confirmed by polymerase chain reaction (PCR). PCR results also indicated that Candidatus Kuenenia stuttgartiensis may be one of the dominant species in the reactor. (C) 2011 Elsevier Ltd. All rights reserved.
Partial nitrification and anammox process: A method for high strength optoelectronic industrial wastewater treatment
(2013-06-01)
Daverey, Achlesh
;
Su, Sin-Han
;
Huang, Yu-Tzu
;
Chen, Shiou-Shiou
;
Sung, Shihwu
Completely autotrophic nitrogen removal over nitrite (CANON) process was employed in an 18 L sequencing batch reactor (SBR) for treatment of optoelectronic industrial wastewater containing high strength ammonium nitrogen (3712 +/- 120 mg NH4+ - N L-1). About 89% of total nitrogen and 98% of NH4+ - N removal efficiencies were observed at the loading rate of 909 g N m(-3) d(-1) and the HRT of 4 d. A profound variation in the performance of CANON process was experienced at high DO exposure (above 1 mg L-1) and high nitrite concentration (above 100 mg L-1). Inhibition due to high DO exposure was found to be reversible phenomenon whereas the synergistic inhibition of nitrite, free ammonia and free nitrous acid was irreversible. The fluctuation of reactor temperature between 17 and 37 degrees C did not affect the performance of CANON system. The CANON process was stably controlled at high nitrogen loading rate for more than one month. The co-existence of aerobic and anaerobic ammonium oxidizing bacteria in the reactor was detected by The PCR analysis. About 5 fold increase in amount of anammox bacteria over a period of 258 days was confirmed from the results of qPCR on day 487. (C) 2013 Elsevier Ltd. All rights reserved.
Performance Analysis for Aggregated Selective Repeat ARQ Scheme in IEEE 802.11n Networks
(2009)
Huang, Yu-Tzu
;
Lin, Jia-Shi
;
Feng, Kai-Ten
;
電子工程學系及電子研究所
;
Department of Electronics Engineering and Institute of Electronics
The next generation wireless local area networks (WLANs) with enhanced throughput performance have attracted significant amounts of attention in recent years. Based on the IEEE 802.11n standard, frame aggregation is considered one of the major factors to improve the system performance of WLANs from the medium access control perspective. In order to fulfill the requirements of the high throughput performance, feasible design of automatic repeat request (ARQ) mechanisms is considered important for providing reliable data transmission. In this paper, an aggregated selective repeat ARQ (ASR-ARQ) algorithm is proposed, which incorporate the conventional selective repeat ARQ scheme with the consideration of frame aggregation. A novel analytical model based on the signal flow graph is established in order to realize the behaviors of ASR-ARQ algorithm. Simulations are also conducted to validate the effectiveness of proposed ASR-ARQ mechanism.
Simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) in a full-scale landfill-leachate treatment plant
(2010-03-15)
Wang, Chih-Cheng
;
Lee, Po-Heng
;
Kumar, Mathava
;
Huang, Yu-Tzu
;
Sung, Shihwu
The occurrence of simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) observed in a single Partially aerated full-scale bioreactor treating landfill-leachate is reported in his paper. At present, the full-scale bioreactor is treating an average leachate flow of 304 m(3) d(-1) with a sludge retention time between 12 and 18 d. The average COD, NH(4)(+)-N and NO(3)(-)-N concentrations at the upstream end of the bioreactor. i.e., influent, are 554, 634 and 3 mg L(-1), respectively; whereas no NO(2)(-)-N is detected in the influent. The percentage removals of COD and NH(4)(+)-N in the bioreactor were 28% and 80%, respectively. A nitrogen mass balance approach was adopted to analyze the performance of SNAD in the full-scale bioreactor. The total nitrogen (TN) removal by combined partial nitrification and anaerobic ammonium oxidation is 68% and the heterotrophic denitrification contributes to 8% and 23% of TN and COD removals, respectively. The red granule in the bioreactor was analyzed by using fluorescence in situ hybridization and polymerase chain reaction. The results of both analytical methods confirm the presence of anaerobic ammonium oxidizing bacteria as the predominant species along with other Planctomycete-like bacteria. Overall, the SNAD process offers the simultaneous removals of nitrogen and COD in the wastewater. (C) 2009 Elsevier B.V. All rights reserved.
Start-up of simultaneous partial nitrification, anammox and denitrification (SNAD) process in sequencing batch biofilm reactor using novel biomass carriers
(2015-08-01)
Daverey, Achlesh
;
Chen, Yi-Chian
;
Dutta, Kasturi
;
Huang, Yu-Tzu
;
Lin, Jih-Gaw
Simultaneous partial nitrification, anammox and denitrification (SNAD) process was started-up in a 2.5 L sequencing batch biofilm reactor (SBBR) using novel biomass carriers. The SNAD process took only 51 d for start-up at nitrogen loading rate (NLR) and organic loading rate (OLR) of 120 and 60 g/m(3)-d, respectively. Long-term stable operation of SNAD process was observed at NLR and OLR of 360 and 180 g/m(3)-d with average total nitrogen and COD removal efficiencies of >88% and >90%, respectively. The values of conversion ratio Y(NO2-+NO3-)/NH4+ remained below 0.11 after the start-up period, which further confirmed the long-term stability of SNAD process. Results of polymerase chain reaction (PCR), qualitative PCR, and scanning electron microscopic (SEM) analysis of sludge samples confirmed the co-existence and enrichment of AOB, anammox bacteria and denitrifying bacteria in the reactor and biofilm formation on to the carriers. (C) 2015 Elsevier Ltd. All rights reserved.
Treatment of semiconductor wastewater using single-stage partial nitrification and anammox in a pilot-scale reactor
(2016-06)
Liang, Ying-Chang
;
Daverey, Achlesh
;
Huang, Yu-Tzu
;
Sung, Shihwu
;
Lin, Jih-Gaw
Start-up of pilot scale reactor to treat real semiconductor wastewater is reported in this study. Semiconductor industrial wastewater stream was characterized as highly alkaline (pH 10) and contained high concentration of H2O2 of 311 +/- 505 mg/L, ammonium nitrogen of 318 +/- 122 NH4+-N mg/L and negligible COD of <10 mg/L. H2O2 was removed by adding enzyme "REYONET F-35" and H2SO4. Before the H2O2-free wastewater is fed to the biological reactor, the pH is adjusted to similar to 7.0. Completely autotrophic nitrogen removal over nitrite (CANON) process was cultivated in a 1.0 m(3) pilot-scale sequencing batch reactor by using enzyme pretreated semiconductor wastewater without temperature control. The nitrogen loading rate was increased step-wise from 94 to 665 g NH4+-N/m(3) d. The average NH4+-N and total nitrogen removal efficiencies were 85.5 and 75.7%, respectively at NLR of 665 g NH4+-N/m(3) d and HRT of 4 d. The pilot reactor was successfully started up and operated for more than 550 d. The CANON was evidenced by the ratio of the effluent nitrite and nitrate to ammonium ratio (eta) of 0.076 during the steady state operation period. PCR results also confirmed the presence of AOB (aerobic ammonia oxidizing bacteria) and anammox (anaerobic ammonia oxidizing) bacteria in the reactor. (C) 2016 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.