Browsing by Author "Jang-Liaw, Nian-Hong"

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A molecular forensic method for identifying species composition of processed marine mammal meats
(2014-03-01)
Chang, Chia-Hao
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Yao, Chiou-Ju
;
Yu, Hsin-Yi
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Liao, Yun-Chih
;
Jang-Liaw, Nian-Hong
We used universal primers designed for the cytochrome oxidase I (CO I) sequence of the order Cetacea and the family Phocidae to prove that meat fritters sold in Taiwan contained meat from two seal, six cetacean, and one pig species. The sequence information for CO I obtained in this study was limited and population genetics data for the eight sampled marine mammalian species was insufficient to deduce where these marine mammals were hunted. Regardless of the geographic origins of the marine mammal flesh, sale and consumption of marine mammals in Taiwan violates the Wildlife Conservation Act. This study provides PCR primers that could enable government testing of suspect meats to curtail the illegal trade in marine mammal products. (C) 2014 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.
Authenticating the use of dried seahorses in the traditional Chinese medicine market in Taiwan using molecular forensics
(2013-09-01)
Chang, Chia-Hao
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Jang-Liaw, Nian-Hong
;
Lin, Yeong-Shin
;
Fang, Yi-Chiao
;
Shao, Kwang-Tsao
Seahorse, which has a unique appearance and exhibits male pregnancy, is a useful component of traditional Chinese medicine (TCM). With the growing demand for TCM, vast amounts of seahorses are harvested from the wild every year and traded internationally. This study investigated 58 dried seahorse samples collected from 23 Chinese herbal medicine stores across Taiwan using molecular forensics. Results showed that eight seahorse species were present in the Taiwan TCM market. Among them, Knysna seahorse (Hippocampus capensis) has an endangered status according to the International Union for Conservation of Nature (IUCN) Red List, while the West African seahorse (Hippocampus algiricus), tiger tail seahorse (Hippocampus comes), thorny seahorse (Hippocampus histrix), great seahorse (Hippocampus kelloggi), yellow seahorse (Hippocampus kuda), hedgehog seahorse (Hippocampus spinosissimus), and three-spot seahorse (Hippocampus trimaculatus) have vulnerable status. Copyright (C) 2013, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. All rights reserved.
Complete mitochondrial genome of the Chinese rasbora Rasbora steineri (Teleostei, Cyprinidae)
(2013-06-01)
Chang, Chia-Hao
;
Tsai, Chi-Li
;
Jang-Liaw, Nian-Hong
;
生物科技學系
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Department of Biological Science and Technology
We describe the complete mitochondrial genome sequence of the Chinese rasbora, Rasbora steineri, which is a small cyprinid distributed in southeastern Asia. The circle genome (16,530 bp) consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. It has the typical vertebrate mitochondrial gene arrangement.
Complete mitochondrial genome of the longfin mako shark, Isurus paucus (Chondrichthyes, Lamnidae)
(2016-01-02)
Chang, Chia-Hao
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Chiang, Wei-Chuan
;
Lin, Yeong-Shin
;
Jang-Liaw, Nian-Hong
;
Shao, Kwang-Tsao
Here we describe the complete mitochondrial genome sequence of the longfin mako, Isurus paucus, which is a pelagic shark found in temperate and tropical waters. The circle genome (16,704bp) consists of 13 protein coding, 22 tRNA, 2 rRNA genes and 1 control region. It has the typical vertebrate mitochondrial gene arrangement.
Complete mitochondrial genome of the megamouth shark Megachasma pelagios (Chondrichthyes, Megachasmidae)
(2014-06-01)
Chang, Chia-Hao
;
Shao, Kwang-Tsao
;
Lin, Yeong-Shin
;
Chiang, Wei-Chuan
;
Jang-Liaw, Nian-Hong
Here we describe the complete mitochondrial genome sequence of the megamouth shark, Megachasma pelagios, which is an extremely rare species of deepwater shark. The circle genome (16,694 bp) consists of 13 protein coding, 22 tRNA, 2 rRNA genes and 1 control region. It has the typical vertebrate mitochondrial gene arrangement.
Complete mitochondrial genome of the Moltrecht's minnow, Aphyocypris moltrechti (Teleostei, Cyprinidae), in comparison with A. kikuchii
(2013-04-01)
Jang-Liaw, Nian-Hong
;
Tsai, Chi-Li
;
Chang, Chia-Hao
;
Watanabe, Katsutoshi
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生物科技學系
We sequenced the complete mitochondrial genome of the Moltrecht's minnow, Aphyocypris moltrechti, which is known as an endemic species to Taiwan. The complete mitochondrial genome is 16,617 bp in size, consisting of 37 genes coding for 13 proteins, 2 rRNAs, 22 tRNAs, and 1 control region. Its gene arrangement pattern was identical with most vertebrates. We compared the mitochondrial genome of A. moltrechti with that of the Kikuchi's minnow, Aphyocypris kikuchii, which had been considered closely related to A. moltrechti within a same genus. Nucleotide sequence divergence (K2P distance) between the two whole mitochondrial genomes was 11.62%. The detailed comparison between the mitochondrial genomes of two species was done.
Complete mitogenomes of two Puntius in Taiwan: P-semifasciolatus and P-snyderi (Cypriniformes: Cyprinidae)
(2013-06-01)
Jang-Liaw, Nian-Hong
;
Chang, Chia-Hao
;
Tsai, Chi-Li
;
生物科技學系
;
Department of Biological Science and Technology
We sequenced the complete mitochondrial genome of two spotted barbs native to Taiwan: Puntius semifasciolatus and Puntius snyderi. The complete mitochondrial genomes are 16,594 and 16,578 bp in size, respectively. Both of them contain 37 genes coding for 13 proteins, 2 rRNAs, 22 tRNAs, and 1 control region. They share the same gene arrangement pattern that was identical with most vertebrates. Nucleotide sequence divergence (K2P distance) between the two whole mitochondrial genomes was 7.63%. These two spotted barbs show very close relationship based on the comparison of the characters of their mitochondrial genomes.
The complete mitochondrial genome of the salmon shark, Lamna ditropis (Chondrichthyes, Lamnidae)
(2016-01-02)
Chang, Chia-Hao
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Jang-Liaw, Nian-Hong
;
Lin, Yeong-Shin
;
Carlisle, Aaron
;
Hsu, Hua Hsun
The complete mitochondrial genome of the salmon shark consists of 16,699bp and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 replication origin region and 1 control region. The mitochondrial gene arrangement of the salmon shark is the same as that of most vertebrates. Base composition of the genome is A (29.6%), T (28.6%), C (27.1%), and G (14.8%).
The complete mitochondrial genome of the tiger tail seahorse, Hippocampus comes (Teleostei, Syngnathidae)
(2013-06-01)
Chang, Chia-Hao
;
Lin, Han-Yang
;
Jang-Liaw, Nian-Hong
;
Shao, Kwang-Tsao
;
Lin, Yeong-Shin
The complete mitochondrial genome of the tiger tail seahorse was sequenced using a polymerase chain reaction-based method. The total length of mitochondrial DNA is 16,525 bp and includes 13 protein-coding genes, 2 ribosomal RNA, 22 transfer RNA genes, and a control region. The mitochondrial gene arrangement of the tiger tail seahorse is also matching the one observed in the most vertebrate creatures. Base composition of the genome is A (32.8%), T (29.8%), C (23.0%), and G (14.4%) with an A + T-rich hallmark as that of other vertebrate mitochondrial genomes.