Browsing by Author "Jhan, Jhen-Wei"

Now showing 1 - 3 of 3

Integrated multiple multi-photon imaging and Raman spectroscopy for characterizing structure-constituent correlation of tissues
(2008-10-13)
Jhan, Jhen-Wei
;
Chang, Wei-Tien
;
Chen, Hung-Che
;
Lee, Yu-Tsung
;
Wu, Ming-Fang
Histopathological imaging of tissues often requires extensive sample preparation including fixation and staining in order to highlight characteristic alterations associated with diseases. Herein, we report an integrated spectro-microscopy approach based on a combination of multimodal multi-photon imaging and Raman micro-spectroscopy and demonstrate label-free characterization of the structure-constituent correlation of porcine skin. The multi-modal imaging allows the visualization of dermatological features whereas Raman micro-spectroscopy enables the identification of their 'molecular fingerprints'. By obtaining both structural and molecular-level information of tissue constituents, this integrated approach can offer new insight into the patho-physiological status of tissues. (c) 2008 Optical Society of America
Optical biopsy of atherosclerotic lesions using integrated second-harmonic and nonlinear Raman microscopy
(2008-06-01)
Chang, Wei-Tien
;
Jhan, Jhen-Wei
;
Wu, Yao-Ming
;
Chen, Hung-Che
;
Liau, Ian
Quantitative Assessment of Hepatic Fat of Intact Liver Tissues with Coherent Anti-Stokes Raman Scattering Microscopy
(2009-02-15)
Wu, Yao-Ming
;
Chen, Hung-Che
;
Chang, Wei-Tien
;
Jhan, Jhen-Wei
;
Lin, Hung-Lung
A fatty liver might progress from being a benign fatty liver, to steatohepatitis, cirrhosis, or even hepatocellular carcinoma. The great prevalence and severe outcome have warranted much investigation of the pathology and the development of effective therapies, which involve animal studies requiring critical evaluation of the hepatic fatty change. Histological examination and wet chemical analysis of liver biopsy specimens are generally employed for this purpose despite numerous procedures being involved. Using coherent anti-Stokes Raman scattering (CARS) microscopy, we have demonstrated the specific imaging of fat droplets in intact liver tissues and extracted the hepatic fat content through image analysis while eliminating laborious procedures required by traditional histopathological. examination. The content of hepatic fat measured with CARS imaging was correlated strongly with that determined by biochemical analysis (R(2) = 0.89) over a pathologically significant range of the hepatic fat (from 2% to 20% of the total mass of tissue). Our work validates the quantitative assessment of fat in intact tissue through the use of CARS microscopy. When combined with the increasingly diverse animal models of diseases related to metabolic disorders of lipids, our approach is extensible to enable acquiring important insight into the genetic, environmental, and dietary factors affecting the uptake and accumulation of fat within tissues.