Browsing by Author "Kakita, Minoru"

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Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy
(2013-03-01)
Kakita, Minoru
;
Okuno, Masanari
;
Hamaguchi, Hiro-o
;
應用化學系
;
應用化學系分子科學碩博班
Raman spectra and images of a living L929 (NCTC) cell have been measured with 532 nm excitation. Both reduced and oxidized forms of cytochromes b and c (cyt b and cyt c) have been observed in situ without any pretreatment. The redox states of cyts b and c have been assessed quantitatively with a spectral analysis. It has been found that reduced cyt c is more abundant than oxidized cyt c, while oxidized cyt b is slightly more abundant than reduced cyt b in a living cell. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Resonance Raman quantification of the redox state of cytochromes b and c in-vivo and in-vitro
(2012-01-01)
Kakita, Minoru
;
Kaliaperumal, Venkatesh
;
Hamaguchi, Hiro-o
;
應用化學系
;
應用化學系分子科學碩博班
We observe the redox state changes with respiration of cytochromes b and c in mitochondria in a living Saccharomyces cerevisiae cell as well as in isolated mitochondria with the very use of Raman microspectroscopy. The possibility of monitoring the respiration activity of mitochondria in vivo and in vitro by Raman microspectroscopic quantification of the cytochrome redox states is suggested. It will lead to a new means to assess mitochondrial respiration activity in vivo and in vitro without using any labelling or genetic manipulation. (C) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)