Browsing by Author "Kang, Yi-Da"

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Multitheragnostic Multi-GNRs Crystal-Seeded Magnetic Nanoseaurchin for Enhanced In Vivo Mesenchymal-Stem-Cell Homing, Multimodal Imaging, and Stroke Therapy
(2015-11-04)
Chen, Po-Jung
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Kang, Yi-Da
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Lin, Chen-Huan
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Chen, San-Yuan
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Hsieh, Chia-Hung
A multifunctional nanoseaurchin probe in which mesoporous silica nanobeads with iron oxide nanoparticles embedded and multi-gold nanorods crystal-seeded are fabricated and labeled with umbilical cord mesenchymal stem cells through endocytosis. This nanoplatform enables efficient magnetic remote-controlled guiding for stem cell homing, and provides dual modalities of photoacoustic imaging and magnetic resonance imaging for in situ tracking and long-term monitoring to achieve therapeutic efficacy.
Neurotensin-Conjugated Reduced Graphene Oxide with Multi-Stage Near-Infrared-Triggered Synergic Targeted Neuron Gene Transfection In Vitro and In Vivo for Neurodegenerative Disease Therapy
(2016-12)
Hsieh, Tsung-Ying
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Huang, Wei-Chen
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Kang, Yi-Da
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Chu, Chao-Yi
;
Liao, Wen-Lin
Delivery efficiency with gene transfection is a pivotal point in achieving maximized therapeutic efficacy and has been an important challenge with central nervous system (CNS) diseases. In this study, neurotensin (NT, a neuro-specific peptide)-conjugated polyethylenimine (PEI)-modified reduced graphene oxide (rGO) nanoparticles with precisely controlled two-stage near-infrared (NIR)-laser photothermal treatment to enhance the ability to target neurons and achieve high gene transfection in neurons. First-stage NIR laser irradiation on the cells with nanoparticles attached on the surface can increase the permeability of the cell membrane, resulting in an apparent increase in cellular uptake compared to untreated cells. In addition, second-stage NIR laser irradiation on the cells with nanoparticles inside can further induce endo/lysosomal cavitation, which not only helps nanoparticles escape from endo/lysosomes but also prevents plasmid DNA (pDNA) from being digested by DNase I. At least double pDNA amount can be released from rGO-PEI-NT/pDNA under NIR laser trigger release compared to natural release. Moreover, in vitro differentiated PC-12 cell and in vivo mice (C57BL/6) brain transfection experiments have demonstrated the highest transfection efficiency occurring when NT modification is combined with external multi-stage stimuli-responsive NIR laser treatment. The combination of neuro-specific targeting peptide and external NIR-laser-triggered aid provides a nanoplatform for gene therapy in CNS diseases.
Ultrafast Plane Wave Imaging Based Pulsed Magnetomotive Ultrasound
(2014-01-01)
Ting, Pei-Hsien
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Kang, Yi-Da
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Chen, San-Yuan
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Li, Meng-Lin
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材料科學與工程學系
Recently, pulsed magnetomotive ultrasound (pMMUS) imaging has been introduced to detect magnetic nanoparticles (MNPs) which are not able to be visualized by conventional ultrasound. However, because of the used magnetic short pulse, the reported pMMUS only can use a single-element ultrasound transducer along with mechanical scanning to perform imaging, which significantly limits the imaging fame rate. To solve this problem, we propose an ultrafast plane wave imaging based pMMUS technique. The ultrafast frame rate of plane wave imaging is fast enough to track the magneto-motion of the excited MNPs during the period of the magnetic pulse being applied. Therefore, the proposed ultrafast plane wave pMMUS is capable of visualizing the dynamic response of the excited MNPs, which is highly correlated to tissue characteristics, to an externally-applied magnetic pulse. In our experiments, ultrafast plane wave imaging with a 5 kHz frame rate was used to implement the pMMUS where the MNP motion induced by an 8-ms magnetic pulse was tracked. The results showed that there were significant differences between the ultrafast plane wave pMMUS images of the phantoms with and without MNPs embedded. In addition, gelatin phantoms with 2%, 4% and 6% gelatin were used to mimic tissues with different elasticity. The dynamic responses of the excited MNPs in the three types of phantoms were distinguishable. Overall, it is demonstrated that the feasibility of our proposed ultrafast plane wave pMMUS imaging technique for the visualization of the magneto-motion and dynamic response of the MNPs under the excitation of a short magnetic pulse. More studies are required to further improve the magneto-motion tracking algorithm and explore the relationship between the dynamic response of the excited MNPs and the tissue viscosity and elasticity.
Ultrafast Pulsed Magnetomotive Ultrasound Imaging of Sentinel Lymph Nodes: Small Animal Study
(2015-01-01)
Huang, Yu-Chun
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Houng, Jieh-Yuan
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Kang, Yi-Da
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Chen, San-Yuan
;
Li, Meng-Lin
Sentinel lymph nodes (SLNs) are the first draining lymph nodes while the metastatic cancer cells are spreading. A noninvasive imaging modality for SLN identification with fine needle aspiration biopsy (FNAB) would be beneficial for staging the axillary lymph nodes without surgical intervention. Here we propose a method - ultrafast pulsed magnetomotive ultrasound (pMMUS) for noninvasive SLN identification, which leverages the MRI superparamagnetic iron oxide nanoparticles (SPIOs) and potentially offers real-time imaging capability for SLN FNAB. Ultrafast pMMUS can localize the SPIOs drained to the SLNs and thus identify the SLNs. Experiments on rats were carried out to verify the feasibility. The SLN was identified in pMMUS images which was confirmed by the methylene blue assay while it is challenging to be localized in B-mode image. Overall, we demonstrated the feasibility of the proposed ultrafast pMMUS for noninvasive SLN identification. Proper SPIO particle size, time window of imaging, and motion-tracking algorithm will be studied to further improve the performance of the proposed method.