Browsing by Author "Kuan, T. C."

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Differentiated immortalized porcine mammary epithelial cells grown on polysulfone hollow fiber provide a potential cell culture system for expression of recombinant proteins
(2010)
Kuan, T. C.
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Sun, Y. L.
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Yen, C. Y.
;
Lin, C. S.
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生物科技學系
Expression of recombinant anticoagulant hirudin in the differentiated cultures of the porcine mammary epithelial cell line SI-PMEC
(2008-07-01)
Sun, Y. L.
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Chou, Y. C.
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Kuan, T. C.
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Tu, C. F.
;
Lin, C. S.
To express recombinant proteins in the spontaneously immortalized porcine mammary epithelial cell line (SI-PMEC) currently established in our laboratory, a chemically synthesized DNA fragment encoding the anticoagulant hirudin was used to construct a mammalian expression vector under the control of the goat beta-casein regulatory sequence. The vector, named pGB562/Hi, was transfected into the SI-PMEC cells to yield pGB562/ Hi/SI-PMEC. The pGB562/Hi/SI-PMEC cells expressed recombinant hirudin only when they were differentiated into functional structures by growth on a Matrigel-coated petri dish supplemented with the lactogenic hormone prolactin. The differentiated pGB562/Hi/SI-PMEC cells produced about 0.5-0.6 mu g of recombinant hirudin/mg of total cellular protein. These results suggest that the established SI-PMEC cells have pharmaceutical potential to inducibly express bioactive heterogeneous proteins. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
The changes of matrix metalloproteinase-2 and-9 enzyme activities in the children with ventricular septal defect
(2013-08-01)
Lin, C. S.
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Cheng, K. S.
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Kuan, T. C.
;
Chen, M. Y.
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生物科技學系