<link rel="stylesheet" href="styles.cf390c6bfbe7555f.css">

Browsing by Author "LI Yaw-Kuen"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item type:Item,
    BORATE MOIETY-CONTAINED LINKER AND BIO-SENSING ELEMENT CONTAINING THE SAME
    (2014-01-02)
    LI Yaw-Kuen
    ;
    SHEN Mo-Yuan
    ;
    PIEN Yu-Ju
    A borate moiety-contained linker and a bio-sensing element containing the same are disclosed. The borate moiety-contained linker can be used to modify a sensing molecule and connect the sensing molecule to a substrate to form the bio-sensing element.
  • Thumbnail Image
    Item type:Item,
    BORATE MOIETY-CONTAINED LINKER AND BIO-SENSING ELEMENT CONTAINING THE SAME
    (2015-04-02)
    LI Yaw-Kuen
    ;
    SHEN Mo-Yuan
    ;
    PIEN Yu-Ju
    A borate moiety-contained linker and a bio-sensing element containing the same are disclosed. The borate moiety-contained linker can be used to modify a sensing molecule and connect the sensing molecule to a substrate to form the bio-sensing element.
  • Thumbnail Image
    Item type:Item,
    METHOD OF PROTEIN PURIFICATION
    (2014-01-30)
    LI Yaw-Kuen
    ;
    WU Yue-Jin
    A method for producing a target protein is provided, which includes steps described below. A crude extract including a fusion protein is provided. The fusion protein includes a tag, a target protein and a linker inserted between the tag and the target protein. The fusion protein and magnetic particles are then bound to form a magnetic particle-binding fusion protein. Finally, the linker of the magnetic particle-binding fusion protein undergoes autocleavage by using a cleavage buffer solution to release the target protein. A one-pot process for producing a purified target protein is also provided.