Browsing by Author "Lai, Lee-Jene"

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Rapid and sensitive detection of cancer cells by coupling with quantum dots and immunomagnetic separation at low concentrations
(2011-06-15)
Hsieh, Yi-Heui
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Lai, Lee-Jene
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Liu, Shih-Jen
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Liang, Keng S.
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電子物理學系
This work presents a rapid and sensitive method for detecting cancer cells at low concentration. In this method, two biomarkers of T-help cancer cells are detected simultaneously. One biomarker is conjugated with magnetic beads to separate T-help cell from the mixed cells and the other biomarker, associated with quantum dots, is used to detect fluorescence. The specific T-help cells can be quantified using the relationship between the QD fluorescence intensity and the cell frequency following magnetic separation. The intensity of fluorescence increases linearly with the frequency of T-help cells from 10(-7) to 10(-3), and neither B cells nor red blood cells interfere with the detection of T-help cells. Moreover, the total detection time is under 15 min, even though the frequency of specific T-help cells is as low as 5 x 10(-7). The numerous advantages of detecting specific cells at low concentration using the presented method include ease of preparation, low cost, fast detection, and high sensitivity. (C) 2011 Elsevier B.V. All rights reserved.
Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
(2012)
Cheng, Hao-Yuan
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Lai, Lee-Jene
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Ko, Fu-Hsiang
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材料科學與工程學系
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Department of Materials Science and Engineering
This study presents a rapid and sensitive method for detecting cancer cells occurring at low concentration. The method involves the simultaneous detection of two biomarkers of T helper cancer cells. One biomarker conjugates with immunofunctionalized magnetic nanoparticles (MNPs), enabling the separation of the T helper cells from a mixed population of cells. The other biomarker is used for detection during enzyme-linked immunosorbent assay (ELISA) analysis. The specific T helper cells can be quantified according to their ELISA absorbance values following magnetic separation. The experimental results demonstrate that immunofunctionalized MNPs can function as magnetic sensors and separate specific T helper cells from a mixed population with high efficiency and high specificity. Coupled with the ELISA technique, the immunofunctionalized MNPs can simultaneously detect rare cells. Results indicated increasing absorbance with increasing T cell number (from 10 to 10(6)). The total detection time was less than 15 minutes, even at a low T cell count. The advantages of the proposed method for detecting specific cells at low concentration include ease of preparation, low cost, rapid detection, and high sensitivity. The proposed system can be adopted to detect circulating tumor cells in early tumor stages for diagnostic or prognostic purposes.
Using Novel Method to Detect Different Cancer-Cell Stages of Model Human Lung Carcinoma
(2015-07-01)
Cheng, Hao-Yuan
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Ko, Fu-Hsiang
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Lai, Lee-Jene
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材料科學與工程學系
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Department of Materials Science and Engineering
BackgroundSynchrotron radiation infrared (SR-IR) microspectroscopy and SR-IR spectroscopic imaging are extremely valuable techniques for determining the molecular composition of biological and biomedical samples. In this work, SR-IR is applied in the study of the lung cancer cells in different cell cycles. MethodsWe use a novel synchrotron based radiation infrared system combined synchronized model human lung carcinoma to reveal its unique character pattern. ResultsAfter using SR-IR microspectroscopy, we discovered that the ratio of protein to lipid in G1 and G2 states is around 4.0 and 6.1, respectively. Moreover, for the DNA at the wavenumber position of 1225 cm(-1), the intensity ratio of G2 state to G1 state is approximately 1.6. These data indicate that the cell in G1 state has more lipid composition to prepare for the DNA synthesis, but the cell in G2 state has more protein composition to prepare for the mitosis. The cell has larger DNA concentration in G2 state, which can be explained for the DNA synthesis. ConclusionThrough our research, we demonstrate that different growth state of cancer cell presenting unique functional groups concentration profiles and distribution via using SR-IR microspectrometry. These applications will provide another ways to improve modern cancer screening in the future.