Browsing by Author "Lee, Ai-Wei"

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CRISPR/Cas9 Genome Editing of Epidermal Growth Factor Receptor Sufficiently Abolished Oncogenicity in Anaplastic Thyroid Cancer
(2018-01-01)
Huang, Li-Chi
;
Tam, Ka-Wai
;
Liu, Wei-Ni
;
Lin, Chun-Yu
;
Hsu, Kai-Wen
Anaplastic carcinoma of the thyroid (ATC), also called undifferentiated thyroid cancer, is the least common but most aggressive and deadly thyroid gland malignancy of all thyroid cancers. The aim of this study is to explore essential biomarker and use CRISPR/Cas9 with lentivirus delivery to establish a gene-target therapeutic platform in ATC cells. At the beginning, the gene expression datasets from 1036 cancers from CCLE and 8215 tumors from TCGA were collected and analyzed, showing EGFR is predominantly overexpressed in thyroid cancers than other type of cancers (P = 0 017 in CCLE and P = 0 001 in TCGA). Using CRISPR/Cas9 genomic edit system, ATC cells with EGFR sgRNA lentivirus transfection obtained great disruptions on gene and protein expression, resulting in cell cycle arrest, cell growth inhibition, and most importantly metastasis turn-off ability. In addition, the FDA-approved TKI of afatinib for EGFR targeting also illustrates great anticancer activity on cancer cell death occurrence, cell growth inhibition, and cell cycle arrest in SW579 cells, an EGFR expressing human ATC cell line. Furthermore, off-target effect of using EGFR sgRNAs was measured and found no genomic editing can be detected in off-target candidate gene. To conclude, this study provides potential ATC therapeutic strategies for current and future clinical needs, which may be possible in increasing the survival rate of ATC patients by translational medicine.
Visualization platform of one-dimensional gratings of tethered polyvinyltetrazole brushes on silicon surfaces for sensing of Cr(III)
(2017-08-01)
Zeng, Jun-Ru
;
Cheng, Chih-Chia
;
Lee, Ai-Wei
;
Wei, Po-Li
;
Chen, Jem-Kun
Polyacrylonitrile (PAN) was grafted on silica (Si) surfaces in the form of one-dimensional (1D) gratings to form well-defined line patterns. The tethered PAN 1D gratings were then subjected to a cyano-to-tetrazole conversion reaction to generate a tethered polyvinyltetrazole (PVT) 1D grating. This results in different properties for the transverse magnetic (TM) and transverse electric (TE) polarizations, i.e. perpendicular and parallel to the line patterns, respectively. After adsorption of Cr(III), the negative charges of PVT chains were neutralized, and this results in a collapse of the polymer brushes. As a result, the effective refractive indices vary with the concentration of Cr(III). This causes a gradual color change from yellow via green to blue at incident angles of 30-40 degrees. The adsorption of the ions Na+, Ag+, Ca2+, Cd2+, Fe3+, Zn2+ and Cr6+ ions, in contrast, does not cause significant changes in the effective refractive index. The Cr(III)-induced deswelling transitions can be reversed by treatment with acid. The assay has a limit of detection (LOD) as low as 20 mu g.L-1 of Cr( III). The ions Pb(II), Cu(II) and Cr(VI) are also adsorbed by the PVT grating, but the respective LODs are higher (80, 100 and 40 mu g.L-1)