Browsing by Author "Lee, I-Ju"

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Confocal Imaging Guided Photochemical Thrombosis Toward the Development of a Novel Zebrafish Model of Stroke
(2016-01-01)
Lee, I-Ju
;
Hsu, Jia-Wen
;
Chuang, Yung-Jen
;
Liau, Ian
;
生物科技學系
Confocal imaging guided photochemical thrombosis was demonstrated on living zebrafish. Occlusion at selected cerebral blood vessels caused varied mortality rate and neurological outcome. This novel zebrafish stroke model may facilitate translational research and thrombolytic screening.
Controllable NO release from Cu1.6S nanoparticle decomposition of S-nitrosoglutathiones following photothermal disintegration of polymersomes to elicit cerebral vasodilatory activity
(2017-01-01)
Kao, Po-Tsung
;
Lee, I-Ju
;
Liau, Ian
;
Yeh, Chen-Sheng
;
交大名義發表
Since the discovery of nitric oxide (NO) as a vasodilator, numerous NO therapies have been attempted to remedy disorders related to pathological vasoconstriction such as coronary artery disease. Despite the advances, clinical applications of NO therapies remain limited mainly because of the low stability of molecular NO donors (and NO molecules), and concerns about the increased oxidative stress and reduced arterial pressure associated with the systemic administration of NO. Here we design a photo-responsive polymersome with nitrosothiols and Cu1.6S nanoparticles in its core and shell, respectively, and demonstrate the photo-triggered release of NO and its vasodilatory activity on zebrafish. Unlike conventional approaches, our design enhances the stability of NO donors and prospectively enables spatiotemporal regulation of NO release, thus minimizing the harmful effects associated with conventional NO therapies. We anticipate that such a strategy will open up new clinical applications of NO and help reveal the complex biological effects of NO in vivo.
Zebrafish model of photochemical thrombosis for translational research and thrombolytic screening in vivo
(2017-04-01)
Lee, I-Ju
;
Yang, Yi-Cyun
;
Hsu, Jia-Wen
;
Chang, Wei-Tien
;
Chuang, Yung-Jen
Acute thromboembolic diseases remain the major global cause of death or disability. Although an array of thrombolytic and antithrombotic drugs has been approved to treat or prevent thromboembolic diseases, many more drugs that target specific clotting mechanisms are under development. Here a novel zebrafish model of photochemical thrombosis is reported and its prospective application for the screening and preclinical testing of thrombolytic agents in vivo is demonstrated. Through photochemical excitation, a thrombus was induced to form at a selected section of the dorsal aorta of larval zebrafish, which had been injected with photosensitizers. Such photochemical thrombosis can be consistently controlled to occlude partially or completely the targeted blood vessel. Detailed mechanistic tests indicate that the zebrafish model of photochemical thrombosis exhibits essential features of classical coagulation and a thrombolytic pathway. For demonstration, tissue plasminogen activator (tPA), a clinically feasible thrombolytic agent, was shown to effectively dissolve photochemically induced blood clots. In light of the numerous unique advantages of zebrafish as a model organism, our approach is expected to benefit not only the development of novel thrombolytic and antithrombotic strategies but also the fundamental or translational research targeting hereditary thrombotic or coagulation disorders.