Browsing by Author "Lee, PH"

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Constructing quantitative models from qualitative mutant phenotypes: preferences in selecting sensory organ precursors
(2006-06-01)
Hsu, CP
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Lee, PH
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Chang, CW
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Lee, CT
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應用化學系分子科學碩博班
Motivation: To study biology from the systems level, mathematical models that describe the time-evolution of the system offer useful insights. Quantitative information is required for constructing such models, but such information is rarely provided. Results: We propose a scheme-based on random searches over a parameter space, according to criteria set by qualitative experimental observations-for inferring quantitative parameters from qualitative experimental results. We used five mutant constraints to construct genetic network models for sensory organ precursor formation in Drosophila development. Most of the models were capable of generating expression patterns for the gene Enhancer of split that were compatible with experimental observations for wild type and two Notch mutants. We further examined factors differentiating the neural fate among cells in a proneural cluster, and found two opposite driving forces that bias the choice between middle cells and the peripheral cells. Therefore, it is possible to build numerical models from mutant screening and to study mechanisms behind the complicated network.
Evaluation of sewage sludge-based compost by FT-IR spectroscopy
(2006-02-01)
Grube, M
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Lin, JG
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Lee, PH
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Kokorevicha, S
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環境工程研究所
The aerobic batch composting fermentations of sewage sludge with wood chips and maturity compost as co-composting additives were carried out in an open type lab-scale reactor. Fourier transform infrared spectroscopy (FT-IR) spectroscopy was used to monitor the composting process, evaluate the degradation rate and thus determine the maturity. Although the composition of the input mixture strongly affects the shape of the infrared (IR) spectra, typical bands of components can be selected and used to follow the composting process. The appearance, shape and intensity of the nitrate band at 1384 cm(-1) was well pronounced and evident for a sewage sludge-based compost maturity. An increase of the peak ratio 1384/2925 and decrease of 2925/1034, 1034/1384 correlated with the degree of decomposition. For the composting mixture under study the peak ratios 1034/1384 and 1384/2925 were more demonstrative. Considering the influence of the composting mixture (components and their ratio) on the shape of the FT-IR spectra the nitrate band at 1384 cm(-1) can be overlapped by other absorption bands (e.g. lignin bands in 1300-1400 cm(-1) region) thus appearing in the spectra as a shoulder, and therefore the ratios 1384/2925 and 1034/1384 become unusable to evaluate the maturity. FT-IR spectroscopy is a quick and useful method to monitor the composting process; however any particular composting mixture needs preliminary studies of the spectra. Consequently, the most appropriate criteria describing the process (e.g. band ratios, shape and intensity of nitrate band) should be selected and the time/peak ratio curves recorded for a compost mixture under study. Afterwards monitoring of the composting process can be based on the comparison of the controls and definite samples. (C) 2005 Elsevier B.V. All rights reserved.