Browsing by Author "Lee, Tzu-Cheng"

Now showing 1 - 5 of 5

Human haptoglobin phenotypes and concentration determination by nanogold-enhanced electrochemical impedance spectroscopy
(2011-05-17)
Cheng, Tsai-Mu
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Lee, Tzu-Cheng
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Tseng, Shin-Hua
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Chu, Hsueh-Liang
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Pan, Ju-Pin
Haptoglobin (Hp) is an acute phase protein that binds free hemoglobin (Hb), preventing Hb-induced oxidative damage in the vascular system. There are three phenotypes in human Hp, whose heterogeneous polymorphic structures and varying concentrations in plasma have been attributed to the cause of diseases and outcome of clinical treatments. Different phenotypes of Hp may be composed of the same subunits but different copy numbers, rendering their determination difficult by a single procedure. In this study, we have developed a simple, fast, reliable and sensitive method, using label-free nanogold-modified bioprobes coupled with self-development electrochemical impedance spectroscopy (EIS). By this method, probe surface charge transfer resistance is detected. The relative charge transfer resistance ratios for Hp 1-1, Hp 2-1 and Hp 2-2 were characterized. We were able to determine protein size difference within 3 nm, and the linear region of the calibration curve for Hp levels in the range of 90 pg ml(-1) and 90 mu g ml(-1) (similar to 1 fM to 1 pM). We surmise that similar approaches can be used to investigate protein polymorphism and altered protein-protein interaction associated with diseases.
Laser Induced Popcorn-like Conformational Transition of Nano-diamond as a Nanoknife
(2009-02-01)
Chang, Chia-Ching
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Chu, Hsueh-Liang
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Lee, Tzu-Cheng
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Chou, Ching-Chung
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交大名義發表
Laser induced popcornlike conformational transition of nanodiamond as a nanoknife
(2008-07-21)
Chang, Chia-Ching
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Chen, Pei-Hsin
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Chu, Hsueh-Liang
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Lee, Tzu-Cheng
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Chou, Ching-Chung
Nanodiamond (ND) is surrounded by layers of graphite on its surface. This unique structure feature creates unusual fluorescence spectra, which can be used as an indicator to monitor its surface modification. Meanwhile, the impurity, nitroso (C - N = O) inside the ND can be photolyzed by two-photon absorption, releasing NO to facilitate the formation of a sp(3) diamond structure in the core of ND and transforming it into a sp(2) graphite structure. Such a conformational transition enlarges the size of ND from 8 to 90 nm, resulting in a popcornlike structure. This transition reaction may be useful as nanoknives in biomedical application. (c) 2008 American Institute of Physics.
Popcorn-Like Conformational Transition of Bio-Nanoparticle Complex: Analysis and Application
(2008-04-01)
Lee, Tzu-Cheng
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Chou, Ching-Chung
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Cheng, Pei-Hsin
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Chang, Chia-Ching
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生物科技學系
ULTRA SENSITIVE DETECTION OF ENETEROVIRUS 71 BY MODIFIED ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY
(2010)
Tseng, Shin-Hua
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Tseng, Dion T.
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Lee, Tzu-Cheng
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Cheng, Tsai-Mu
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Yang, Jyh-Yuan
The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (similar to sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.