Browsing by Author "Li, Shu-Ying"

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Association between fluconazole susceptibility and genetic relatedness among Candida tropicalis isolates in Taiwan
(2007-05-01)
Wang, Jang-Shiun
;
Li, Shu-Ying
;
Yang, Yun-Liang
;
Chou, Hsiao-Hui
;
Lo, Hsiu-Jung
Among the 162 Candida tropicalis isolates Collected in the Taiwan Surveillance of Antimicrobial Resistance of Yeasts in 1999, 23 (14.2%) had fluconazole MICs >= 64 mg l(-1), and thus fulfilled the definition of resistance. Random amplified polymorphic DNA assay showed that all 23 fluconazole-resistance C. tropicalis isolates collected from different hospitals around Taiwan were closely related. Two distinct pulsotypes associated with fluconazole susceptibility were identified when these 23 resistant isolates, along with 13 susceptible ones, were analysed by PFGE.
Susceptibilities to amphotericin B and fluconazole of Candida species in Taiwan Surveillance of Antimicrobial Resistance of Yeasts 2006
(2008-06-01)
Yang, Yun-Liang
;
Wang, An-Huei
;
Wang, Chih-Wei
;
Cheng, Wei-Ting
;
Li, Shu-Ying
Susceptibilities to amphotericin B and fluconazole of 964 Candida isolates collected in Taiwan Surveillance of Antimicrobial Resistance of Yeasts in 2006 were determined. There were 419 (43.5%) Candida albicans, 246 (25.5%) Candida tropicalis, 211 (21.9%) Candida glabrata, 62 (6.4%) Candida parapsilosis, 14 (1.5%) Candida krusei, and 12 (1.2%) others. Interestingly, 16 of the 17 amphotericin B-resistant isolates were non-albicans Candida species. The resistant rate to amphotericin B has decreased from 2.5% in 2002 to 1.8% in 2006. On the other hand, there were 132 C. tropicalis, 14 C. krusei, 10 C. albicans, and 9 C. glabrata isolates that had MICs to fluconazole >= 64 mu g/mL. The prevalence of isolates with such high MICs was significantly higher than that in 2002 (17.1% versus 1.9%). Our results further indicate that most of the isolates with MICs to fluconazole >= 64 mu g/mL exhibited the "trailing" phenomenon. (C) 2008 Elsevier Inc. All rights reserved.
Two Closely Related Fluconazole-Resistant Candida tropicalis Clones Circulating in Taiwan from 1999 to 2006
(2009-09-01)
Li, Shu-Ying
;
Yang, Yun-Liang
;
Lin, Yu-Hsin
;
Ko, Hui-Ching
;
Wang, An-Huei
Recently, we reported that diploid sequence type (DST) 140 was a predominant type of Candida tropicalis among isolates with fluconazole minimum inhibitory concentrations (MICs) >= 64 mu g/ml collected in the Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) in 1999. To determine if DST140 persists in Taiwan, we have used multilocus sequence typing to characterize the genetic profiles of 31 resistant isolates (MICs >= 64 mu g/ml), together with 19 susceptible isolates (MICs <= 16 mu g/ml) collected in TSARY 2006. Among the 50 isolates, 33 distinct DSTs were detected. Of the 31 resistant isolates, 11 (35.5%) belonged to two closely related DSTs (140 and 98), whereas none of the 19 susceptible isolates did (p = 0.004). The isolates belonging to DST140 and 98 were from different geographic regions instead of a restricted area. Thus, our data show temporal and spatial transmission of C. tropicalis clones with high fluconazole MICs in Taiwan from 1999 to 2006.