Browsing by Author "Li, Y. -K."

Now showing 1 - 5 of 5

A marcrolide glucosyltransferase promoting the synthesis of O-, S- and N-glucosidic compounds
(2012-09-01)
Li, Y. -K.
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交大名義發表
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National Chiao Tung University
"A marcrolide glucosyltransferase promoting the synthesis of O-, S- and N-glucosidic compounds"
(2012-09-01)
Li, Y. -K.
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交大名義發表
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National Chiao Tung University
A novel streptococci-conserved beta-lactamase involved in ampicillin resistance of Streptococcus pneumoniae
(2015-07-01)
Li, Y. -K.
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Chang, C. -Y.
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Li, B. -R.
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交大名義發表
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應用化學系
A possibility of detection of the non-charge based analytes using ultra-thin body field-effect transistors
(2008-07-15)
Sheu, J. -T.
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Chen, C. C.
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Chang, K. S.
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Li, Y. -K.
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材料科學與工程學系奈米科技碩博班
Ultra-thin body of p-type field-effect transistors were developed as transducer for biosensors. Changes of conductance resulted from the changes of the surface potentials of ultra-thin body field-effect transistors (UTB-FETs) due to surface chemical modifications were demonstrated. The channel surface of UTB-FETs were modified with N-[3-(trimethoxysilyl)propyl]ethylenediamine (AEAPTMS) and then gold nanoparticles (AuNPs) to immobilize the bio-component, the genetically engineered Delta(5)-3-ketosteroid isomerase (Art_KSI) or the Art_KSI conjugated with charged reporter (Art_KSI_mA51). The binding of charge-based molecules or nanoparticles has been demonstrated to strongly affect the conductivity of UTB-FETs: the increase or decrease of the conductance depends on the polarity of the immobilized molecules or nanoparticles. A new protocol involving the detection of a non-charged analyte relied on the competitive binding of analyte (19-norandrostendione) and a charged reporter (mA51) with KSL When exposed to a 19-norandrostendione solution (10 mu M), the conductance of Art_KSI_mA51-modified UTB-FET increased by 265 nS (similar to 12%). On the other hand, conductance of Art_KSI_modified UTB-FET showed no distinct change under the same detection conditions. (c) 2008 Elsevier B.V. All rights reserved
Mechanistic and structural study of BcGT
(2013-07-01)
Li, Y. -K.
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Chiu, H. -H.
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Wang, S.
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交大名義發表
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National Chiao Tung University