Browsing by Author "Lin, C. S."

Now showing 1 - 9 of 9

A regulatory element within the promoter of angiotensin-converting enzyme 2 (ace2) gene responsible for angiotensin II stimulation
(2012-03-15)
Kuan, T-C.
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Chen, M-C.
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Yang, T-H.
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Wu, W-T.
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Lin, C. S.
Activity and expression profiling of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) in the hearts with doxorubicin-induced cardiomyopathy
(2012-03-15)
Lin, C. S.
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Kuan, T-C.
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Lai, S-C.
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Chen, M-Y.
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Wu, H-T.
Curcumin and genistein inhibit TNF-alpha-inducted matrix metalloproteinase-9 (MMP-9) expression by repressing NF-kappa B-mediated transcription
(2012-03-15)
Wu, H-T
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Sie, S-S
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Chen, C-Y
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Kuan, T-C
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Lin, C. S.
Differentiated immortalized porcine mammary epithelial cells grown on polysulfone hollow fiber provide a potential cell culture system for expression of recombinant proteins
(2010)
Kuan, T. C.
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Sun, Y. L.
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Yen, C. Y.
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Lin, C. S.
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生物科技學系
Establishment and characterization of a spontaneously immortalized porcine mammary epithelial cell line
(2006-12-01)
Sun, Y. L.
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Lin, C. S.
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Chou, Y. C.
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生物科技學系
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Department of Biological Science and Technology
We have established a spontaneously immortalized porcine mammary epithelial cell line (SI-PMEC) from the mammary gland of a lactating sow and maintained it long-term in culture by continuous subculturing. SI-PMEC cells were maintained for more than 8 months (70 passages) in DMEM/F12 medium supplemented with 10% fetal calf serum, insulin, and hydrocortisone without obvious signs of senescence. When grown at low density on a plastic substratum, SI-PMEC cells formed islands, and when grown to confluency, the cells formed a monolayer and aggregated with the characteristic cobblestone morphology of epithelial cells. The subcultured SI-PMEC cells appeared to proliferate without changes in morphology or growth pattern, with an estimated population doubling time of 20-22 h. With increasing density, SI-PMEC cells organized into lumen-like structures with elongated cells at the margins. SI-PMEC cells from stocks frozen at Passage 30 were subcultured up to 20 times without changes in cell viability, proliferation rate, or morphology. Furthermore, SI-PMEC cells remained immunopositive to an antibody against cytokeratin AE3 and immunonegative to an antibody against a human fibroblast antigen. The SI-PMEC cells could form functional structures resembling ducts, lateral-buds, and alveoli in a Matrigel matrix-dependent manner in vitro. When grown on the Matrigel and stimulated by prolactin, the cells differentiated and formed mammary gland structures and strongly expressed transcripts encoding the milk proteins alpha-lactalbumin, beta-casein, and beta-lactoglobulin. Our results indicate that the SI-PMEC cell line can be subpassaged many times and still form functional differentiated secretory structures. To our knowledge, this is the first report of an immortalized mammary epithelial cell line from pig. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Expression of recombinant anticoagulant hirudin in the differentiated cultures of the porcine mammary epithelial cell line SI-PMEC
(2008-07-01)
Sun, Y. L.
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Chou, Y. C.
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Kuan, T. C.
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Tu, C. F.
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Lin, C. S.
To express recombinant proteins in the spontaneously immortalized porcine mammary epithelial cell line (SI-PMEC) currently established in our laboratory, a chemically synthesized DNA fragment encoding the anticoagulant hirudin was used to construct a mammalian expression vector under the control of the goat beta-casein regulatory sequence. The vector, named pGB562/Hi, was transfected into the SI-PMEC cells to yield pGB562/ Hi/SI-PMEC. The pGB562/Hi/SI-PMEC cells expressed recombinant hirudin only when they were differentiated into functional structures by growth on a Matrigel-coated petri dish supplemented with the lactogenic hormone prolactin. The differentiated pGB562/Hi/SI-PMEC cells produced about 0.5-0.6 mu g of recombinant hirudin/mg of total cellular protein. These results suggest that the established SI-PMEC cells have pharmaceutical potential to inducibly express bioactive heterogeneous proteins. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
In vitro studies of repair efficiency of mesenchymal stem cells by co-culture with fibrosis hepatocytes
(2018-07-01)
Chen, H. C.
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Lin, C. S.
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Wu, H. T.
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生物科技學系
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Department of Biological Science and Technology
Regulation of angiotensin converting enzyme II by angiotensin peptides in human cardiofibroblasts
(2010-09-01)
Lin, C. S.
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Yang, T. H.
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Wen, C. H.
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Pan, C. H.
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交大名義發表
The changes of matrix metalloproteinase-2 and-9 enzyme activities in the children with ventricular septal defect
(2013-08-01)
Lin, C. S.
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Cheng, K. S.
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Kuan, T. C.
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Chen, M. Y.
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生物科技學系