Browsing by Author "Lin, Chi-Hung"

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Hydrothermally Roughened Surface-Enhanced Raman Scattering-Active Substrates with Low Background Signals for Chemical Sensing Application
(2011-03-01)
Yang, Jung-Yen
;
Cheng, Hui-Wen
;
Chen, Yu
;
Li, Yiming
;
Lin, Chi-Hung
A low-cost, environment friendly and easily fabricated SERS-active substrate with low background signals has been developed by hydrothermal treatment of titanium thin films deposited on silicon substrate. Results from the experimental study revealed that Rhodamine 6G can be well resolved using gold-coated hydrothermally roughened TiO(2) substrates. The effect of surface enhancement by the hydrothermally roughened substrates enables rapid identification of Rhodamine 6G, and the enhancement capability is controllable by tuning the surface roughness of the substrates through varying treatment duration. The hydrothermally treated TiO(2) substrates also exhibit tunable wettability like that of the untreated ones.
IGDB.NSCLC: integrated genomic database of non-small cell lung cancer
(2012-01-01)
Kao, Sen
;
Shiau, Cheng-Kai
;
Gu, De-Leung
;
Ho, Chun-Ming
;
Su, Wen-Hui
Lung cancer is the most common cause of cancer-related mortality with more than 1.4 million deaths per year worldwide. To search for significant somatic alterations in lung cancer, we analyzed, integrated and manually curated various data sets and literatures to present an integrated genomic database of non-small cell lung cancer (IGDB. NSCLC, http://igdb.nsclc.ibms.sinica.edu.tw). We collected data sets derived from hundreds of human NSCLC (lung adenocarcinomas and/or squamous cell carcinomas) to illustrate genomic alterations [chromosomal regions with copy number alterations (CNAs), gain/loss and loss of heterozygosity], aberrant expressed genes and microRNAs, somatic mutations and experimental evidence and clinical information of alterations retrieved from literatures. IGDB. NSCLC provides user friendly interfaces and searching functions to display multiple layers of evidence especially emphasizing on concordant alterations of CNAs with co-localized altered gene expression, aberrant microRNAs expression, somatic mutations or genes with associated clinicopathological features. These significant concordant alterations in NSCLC are graphically or tabularly presented to facilitate and prioritize as the putative cancer targets for pathological and mechanistic studies of lung tumorigenesis and for developing new strategies in clinical interventions.
Rapid identification of nicotine in electronic cigarette liquids based on surface-enhanced Raman scattering
(2020-01-01)
Chien, Jun-Yi
;
Gu, Yong-Chun
;
Tsai, Hsin-Mei
;
Liu, Chun-Hao
;
Yen, Chia-Yuan
Nicotine-containing electronic cigarette liquid (e-liquid) is prohibited in many countries, creating requirements for rapid detection approaches for on-site inspection or screening for large amounts of samples. Here, we demonstrate a simple way to identify nicotine using surface-enhanced Raman scattering (SERS) with substrates made of silver nanoparticle arrays imbedded in anodic aluminum oxide nanochannels (Ag/AAO). Compared with the reported colloidal nanoparticle-based SERS, that required serial dilutions to enable colloid aggregation in the viscous e-liquid, a small amount of undiluted e-liquid sample can be directly added onto our solid-phase Ag/AAO substrate without any pre-treatment. The sensitivity of our SERS measurements is 2-3 orders of magnitude higher than that required for identification of nicotine in e-liquid, which is typically around 1000-18,000 ppm. Using such nanoparticle array-based SERS, we have tested 22 commercially available e-liquid products, using the corresponding gas chromatography-mass spectrometry (GC-MS) reports as the reference. The SERS measurements were done within one hour and successfully identified 20 samples. Only 2 samples showed SERS interference from ingredients that were not suitable for SERS analysis.
Stepped changes of monovalent ligand-binding force during ligand-induced clustering of integrin alpha(IIB)beta(3)
(2006-09-01)
Hsieh, Chia-Fen
;
Chang, Bo-Jui
;
Pai, Chyi-Huey
;
Chen, Hsuan-Yi
;
Tsai, Jin-Wu
Recent evidence demonstrated that conformational changes of the integrin during receptor activation affected its binding to extracellular matrix; however, experimental assessment of ligand-receptor binding following the initial molecular interaction has rarely been carried out at a single-molecule resolution. In the present study, laser tweezers were used to measure the binding force exerted by a live Chinese hamster ovary cell that expressed integrin alpha(IIb)beta(3) (CHO alpha(IIb)beta(3)), to the bead carrier coated with the snake venom rhodostomin that served as an activated ligand for integrin alpha(IIb)beta(3). A progressive increase of total binding force over time was noticed when the bead interacted with the CHO alpha(IIb)beta(3) cell; such an increase was due mainly to the recruitment of more integrin molecules to the bead-cell interface. When the binding strength exerted by a single ligand-receptor pair was derived from the "polyvalent" measurements, surprisingly, a stepped decrease of the "monovalent binding force" was noted (from 4.15 to 2.54 piconewtons (pN)); such decrease appeared to occur during the ligand-induced integrin clustering process. On the other hand, the mutant rhodostomin defective in clustering integrins exhibited only one (1.81 pN) unit binding strength.