Browsing by Author "Lin, Chih-Hsiang"

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CHARACTERISTIC COLOR USE IN DIFFERENT FILM GENRES
(2012)
Chen, I-Ping
;
Wu, Fang-Yi
;
Lin, Chih-Hsiang
;
應用藝術研究所
;
Institute of Applied Arts
The purpose of this study was to develop a color analysis protocol for studying use of colors in films. The typical color use in films of romance, comedy, horror, sci-fi, and action genres was evaluated. A custom-made software was used for extracting contrast, saturation, brightness, red, green, yellow and blue values from all sampled frames. We found that among the chosen five film categories, patterns of coloring could be divided into two groups-one comprised romance and comedy, and the other horror, sci-fi, and action. Colors in romance and comedy tend to have higher contrast and brightness and are rich in red and yellow while in horror, sci-fi and action it is the reverse. Sci-fi films usually show the highest average standard deviation of brightness, as well as red, green, yellow, and blue among these five film categories. This indicates that colors of sci-fi films tend to vary greatly as the story progresses. The color analysis protocol developed in this study can be a valuable tool for exploring the aesthetic functions of colors in films.
Development of a novel engineered antibody targeting Neisseria species
(2017-03)
Liu, Chih-Yueh
;
Weng, Chang-Ching
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Lin, Chih-Hsiang
;
Yang, Chiou-Ying
;
Mong, Kwok-Kong Tony
A Neissaria bacterial pilus sugar, bacillosamine, was synthesized and, for the first time, used as a probe to screen a single-chain variable fragment (scFv). Four Neisseria, Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria sicca and Neisseria subflava, and two negative controls, Streptococcus pneumoniae and Escherichia coli, were tested through ELISA, immunostaining and gold nanoparticle immunological assay. All results indicated that the selected scFv is feasible for the specific detection of Neisseria species via the recognition of bacillosamine. The recombinant scFv could detect Neisseria strains at 10(6) CFU/ml.
Time-Gated Imaging on Live Cancer Cells Using Silicon Quantum Dot Nanoparticles with Long-Lived Fluorescence
(2017-06-01)
Tu, Chang-Ching
;
Awasthi, Kamlesh
;
Chen, Kuang-Po
;
Lin, Chih-Hsiang
;
Hamada, Morihiko
In this work we demonstrate time-gated confocal fluorescence imaging on live cancer cells immunostained by antibody-conjugated silicon quantum dot nano particles (SiQD-NPs) and organic dyes, for simultaneous detection of two biological targets and removal of background autofluorescence. With almost all radiative recombinations occurring through oxide-related defect states located on the SiQD surface, the SiQD-NPs have very long photoluminescence lifetimes of about 25 its, in contrast to the nanosecond range lifetimes of other commonly used biological fluorophores. This drastic lifetime difference enables a time-gated imaging method here, in which the time-resolved photon distribution of each pixel of a fluorescence image is measured by using a time-correlated single-photon counting technique. Then, by integrating the photon histogram of each pixel over respective time windows, the long-lived component of the fluorescence image comprising only the fluorescence emitted from the SiQD-NPs is separated from all other short-lived signals resulting from the organic dyes and the cell endogenous luminescence. For instance, the membrane and nucleus of a single cancer cell or two types of cancer cells, immunostained with the SiQD-NPs and the organic dyes, respectively, can be clearly distinguished from each other by time-gating, which otherwise cannot be accomplished by conventional multiplexing due to spectral overlap in the wavelength domain.