Browsing by Author "Liou, Ci-Hong"

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Ndt80p is involved in L-sorbose utilization through regulating SOU1 in Candida albicans
(2015-01-01)
Lo, Hsiu-Jung
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Chu, Wen-Li
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Liou, Ci-Hong
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Huang, Szu-Hsuan
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Khoo, Kay-Hooi
Ndt80p, a known transcriptional factor, regulates various targets involved in stress responses, filamentous growth, and virulence in Candida albicans. Potential targets of Ndt80p have been identified at the transcriptional level. The present study was conducted to identify genes regulated by Ndt80p from the protein level. We found that the levels of Ahp1p, Fma1p, Hsp21p, Rfa2p, Snz1p, Sod1p, Soul p, Trp99p, orf19.251, orf19.1862, and orf19.5620, were affected by the null mutation of NDT80 by two-dimensional polyacrylamide gel-electrophoresis analysis. Among the 11 proteins, all but Sou1p and Rfa2p are suggested to be involved in known functions of Ndt80p. Here, we demonstrate that Ndt80p plays a role in t-sorbose utilization through regulating SOU1 in C. albicans. (C) 2014 Elsevier GmbH. All rights reserved.
Rep1p negatively regulating MDR1 efflux pump involved in drug resistance in Candida albicans
(2009-09-01)
Chen, Chia-Geun
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Yang, Yun-Liang
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Tseng, Kuo-Yun
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Shih, Hsin-I
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Liou, Ci-Hong
Overexpression of MDR1 efflux pump is a major mechanism contributing to drug resistance in Candida albicans, the most common human fungal pathogen. To elucidate the regulatory pathway of drug resistance, we have identified a negative regulator of MDR1 and named it Regulator of Efflux Pump 1 (REP1). Overexpression of REPI in Saccharomyces cerevisiae increased susceptibility to fluconazole. Furthermore, null mutations on REP1 decreased the susceptibility to antifungal drugs in C albicans resulting from increased expression of MDR1 mRNA. Hence, Rep1p is involved in drug resistance by negatively regulating MDR1 in C albicans. (c) 2009 Elsevier Inc. All rights reserved.