Browsing by Author "Liu, Heng-Wen"

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Arrayed rGOSH/PMASH Microcapsule Platform Integrating Surface Topography, Chemical Cues, and Electrical Stimulation for Three-Dimensional Neuron-Like Cell Growth and Neurite Sprouting
(2014-06-01)
Liu, Heng-Wen
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Huang, Wei-Chen
;
Chiang, Chih-Sheng
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Hu, Shang-Hsiu
;
Liao, Chia-Hsin
The biocompatible thiol-functionalized rGOSH/PMASH microcapsules encapsulating nerve growth factor (NGF) are arrayed onto a transparent and conductive substrate, i.e., indium tin oxide (ITO), to integrate electrically stimulated cellular differentiation, electrically controlled NGF release, and topographically rough nano-surfaces into a 3-D platform for nerve regeneration. The rGOSH/PMASH microcapsules with microscale topography function not only as an adhesive coating to promote the adhesion of PC12 cells but also as electroactive NGF-releasing electrodes that stimulate NGF release and accelerate the differentiation of PC12 cells during electrical stimulation. Once electrical treatment is applied, NGF release and electrically enhanced cellular differentiation lead to an obvious increase both in the percentage of cells with neurites and in the neurite length. This length can reach nearly 90 m within 2 days of cell culture. The average neurite length is significantly increased (four-fold) after culture on the rGOSH/PMASH microcapsule substrate for 2 days compared with culture on a substrate without an rGOSH/PMASH coating. These multifunctional rGOSH/PMASH microcapsules may be used as potential 3-D patterned substrates for neural regeneration and neural prosthetics in tissue engineering applications.
Characterization and drug release behavior of highly responsive chip-like electrically modulated reduced graphene oxide-poly(vinyl alcohol) membranes
(2012)
Liu, Heng-Wen
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Hu, Shang-Hsiu
;
Chen, Yu-Wei
;
Chen, San-Yuan
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材料科學與工程學系
"Hybrid hydrogel membranes composed of reduced graphene oxide (rGO) nanosheets and a poly (vinyl alcohol) (PVA) matrix were investigated as an electrically responsive drug release system. The rGO nanosheets in the matrix act as a physical barrier to inhibit the release of the model anesthetic drug, lidocaine hydrochloride, but the highly responsive release can be enhanced by the presence of rGO when exposed to an electrical stimulus. More interestingly, the on-demand drug release profiles of the assembled rGO-PVA hydrogel into a chip-like device can be highly controlled by an external electrical field. Release profiles ranging from a slow-elution pattern to a rapid release under electrical field treatment can be achieved by manipulating the rGO-PVA composition. Moreover, under cyclic exposure to an electrical stimulus, a highly controllable and repeatedly pulsatile release with desirable precision is obtained from the rGO-PVA hydrogel, implying that the hydrogel exhibits excellent anti-fatigue properties. By combining with the enhanced structural integrity and biocompatibility, the electrically responsive rGO-PVA hydrogel has demonstrated potential biological applications in drug delivery systems. In addition, the easy loading of hydrophilic drugs into the rGO-PVA hydrogel opens up a promising future in biological applications, such as transdermal therapy and wound healing."