Browsing by Author "Liu, PF"

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Proton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mung bean seedlings
(2005-02-01)
Kuo, SY
;
Chien, LF
;
Hsiao, YY
;
Van, RC
;
Yan, KH
Endoplasmic reticulurn (ER)-enriched vesicles from etiolated hypocotyts of mung bean seedlings (Vigna radiata) were successfully isolated using Ficoll gradient and two-phase (polyethylene glycol-dextran) partition. The ER-enriched vesicles contained inorganic pyrophosphate (PPi) hydrolysis and its associated proton translocating activities. Antiserum prepared against vacuolar H+-pyrophosphatase (V-PPase, EC 3.6.1.1) did not inhibit this novel pyrophosphatase-dependent proton translocation, excluding the possible contamination of tonoptast vesicles in the ER-enriched membrane preparation. The optimal ratios of Mg2+/PPi (inorganic pyrophosphate) for enzymatic activity and PPj-dependent proton translocation of ER-enriched vesicles were, higher than those - of vacuolar membranes. The PPj-dependent Proton translocation of ER-enriched vesicles absolutely required the presence of monovalent cations with preference for K but could be inhibited by a common PPase inhibitor, F-. Furthermore, ER H+-pyrophosphatase exhibited some similarities and differences to vacuolar H+-PPases in cofactor/substrate ratios, pH profile, and concentration dependence of F-, imidodiphosphate (a PPj anatogue), and various chemical modifiers.