Browsing by Author "Liu, Yi-Ju"

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Biomass and lipid production of a novel microalga, Chlorellaceae sp P5, through heterotrophic and swine wastewater cultivation
(2018-05-01)
Chiang, Yin-Ling
;
Chen, Yu-Pei
;
Yu, Ming-Chen
;
Hsieh, Sung-Yuan
;
Hwang, Ing-Er
A potential novel genus of microalgae was isolated from swine wastewater. According to a phylogenetic analysis of 18S rRNA and internal transcribed spacer (ITS) sequences, as well as the ITS2 secondary structure, this strain was designated as Chlorellaceae sp. P5. Acclimatization to various temperatures, pH conditions, and salinities demonstrated that this strain can grow at temperatures of 20 degrees C-40 degrees C, in an acidity range of pH 4-7, and with salinity as high as 3% NaCl. To optimize biomass production, response surface methodology was used which included variables associated with carbon and nitrogen sources. In this study, flask cultivation yielded 1.66 g l(-1) d(-1) of biomass under 15.19 g l(-1) glucose and 8.26 mM ammonium bicarbonate. The fatty acid composition suggested that the lipid from Chlorellaceae sp. P5 can be typically used for biodiesel production, with a low degree of unsaturated fatty acids. Moreover, a high content of the first-limiting amino acid (lysine) and 9,12-Octadecadienoic acid (linoleic acid, C18:2) for nutritional supplements was also observed. With respect to swine wastewater treatment, the isolated strain showed an excellent removal rate of ammonia and phosphate over 97.3 mg l(-1) of NH3-N and 20.3 mg of l(-1) PO43- in the sterilized and none-sterilized wastewater. The biomass and lipid derived from Chlorellaceae sp. P5 can be attained at 0.49 g l(-1) d(-1) and 0.13 g l(-1) d(-1) by swine wastewater cultivation with 5% CO2 supply, respectively. According to the various characteristic analyses, Chlorellaceae sp. P5 has great potential for different industrial developments such as biodiesel and wastewater treatment. Published by AIP Publishing.
Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7
(2008-12-01)
Liu, Yi-Ju
;
Yao, Da-Jeng
;
Chang, Hwan-You
;
Liu, Chien-Ming
;
Chen, Chih
This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis. (C) 2008 Elsevier B.V. All rights reserved.