Browsing by Author "Liu, Yung-Hsin"

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Aerosol-Assisted Plasma Deposition of Biocomposite Coatings: Investigation of Processing Conditions on Coating Properties
(2016-12)
Hsiao, Chun-Ping
;
Wu, Cheng-Chen
;
Liu, Yung-Hsin
;
Yang, Yi-Wei
;
Cheng, Yun-Chien
The aerosol-assisted atmospheric-pressure planar dielectric-barrier-discharge-type plasma deposition (AA-APPD) system can polymerize monomers and immobilize biomolecules simultaneously. In this paper, we used this technology to deposit biocomposite thin films of polyethylene (PE) along with lysozyme (Lyz) under different aerosol solution temperatures and at different coating positions in the discharge region. In addition, we compared the deposition efficacy of two kinds of Lyz solution using deionized (DI)-water and phosphate-buffered saline (PBS) as solvents, respectively. The results show that deposition rate of thin films increases with increasing solution temperature at most coating positions. In addition, coatings from Lyz-PBS perform higher deposition rates, but with weaker Lyz viability as compared with that of Lyz DI-water. Finally, we also verified that this DBD-type AA-APPD system can not only successfully deposit Lyz in PE thin film, but also maintain Lyz viability after plasma process under different solution temperatures and positions.
Aerosol-Assisted Plasma Deposition of Biocomposite Coatings: Investigation of Processing Conditions on Coating Properties
(2016-12-01)
Hsiao, Chun-Ping
;
Wu, Cheng-Chen
;
Liu, Yung-Hsin
;
Yang, Yi-Wei
;
Cheng, Yun-Chien
The aerosol-assisted atmospheric-pressure planar dielectric-barrier-discharge-type plasma deposition (AA-APPD) system can polymerize monomers and immobilize biomolecules simultaneously. In this paper, we used this technology to deposit biocomposite thin films of polyethylene (PE) along with lysozyme (Lyz) under different aerosol solution temperatures and at different coating positions in the discharge region. In addition, we compared the deposition efficacy of two kinds of Lyz solution using deionized (DI)-water and phosphate-buffered saline (PBS) as solvents, respectively. The results show that deposition rate of thin films increases with increasing solution temperature at most coating positions. In addition, coatings from Lyz-PBS perform higher deposition rates, but with weaker Lyz viability as compared with that of Lyz DI-water. Finally, we also verified that this DBD-type AA-APPD system can not only successfully deposit Lyz in PE thin film, but also maintain Lyz viability after plasma process under different solution temperatures and positions.
Enhancing adhesion and polymerization of lipase-plasma-polymerized-ethylene coatings deposited with planar dielectric-barrier-discharge-type aerosol-assisted atmospheric-pressure plasma system
(2018-04-01)
Cheng, Yun-Chien
;
Hsiao, Chun-Ping
;
Liu, Yung-Hsin
;
Yang, Chu-Hao
;
Chiang, Chun-Yi
To enhance the adhesion and polymerization of lipase-plasma-polymerized-ethylene (lipase-PPE) coatings deposited by aerosol-assisted atmospheric plasma deposition (AAAPPD), the effects of voltage, ceramic-dielectric thickness, and pre-coated PPE layer were investigated. In AAAPPD system, ethylene and lipase-phosphate-buffered-saline aerosol were used as precursors to polymerize ethylene and embed lipase simultaneously. The deposited lipase-PPE coatings were analyzed with FTIR, SEM, and surface profiler. The results show that increasing voltage from 3 to 4 kV and decreasing ceramic-dielectric thickness from 1 to 0.38 mm improved the coating adhesion and polymerization. Also, depositing a PPE layer with high energy prior to lipase-PPE coating enhanced adhesion. These improvements of the biomolecule-PPE coatings facilitate further bioapplications of this new biomolecule-embedding method.
Using aerosol-assisted atmospheric-pressure plasma to embed proteins onto a substrate in one step for biosensor fabrication
(2018-09-01)
Liu, Yung-Hsin
;
Yang, Chu-Hao
;
Lin, Tsung-Rong
;
Cheng, Yun-Chien
;
機械工程學系
This study shows that aerosol-assisted dielectric-barrier-discharge atmospheric-pressure plasma deposition (AAAPPD) is a rapid and reagent-free method to entrap proteins on different substrates for biosensor fabrication, and the proteins were not damaged. AAAPPD involves depositing plasma-polymerized ethylene (ppE) with grafted hydroxyl functional groups and embedding the protein in the ppE in one step, making the protein entrapment faster than conventional methods and without using reagents. In this study, SEM and XPS show that more protein deposited near the inlet. Electrophoresis, temperature analysis, and immunostaining respectively demonstrated that the protein maintained its integrity, was unheated, and exhibited activity during AAAPPD. The immunostaining results of AAAPPD protein was close to that of covalent-bonded protein. AAAPPD also entraps protein on three different biosensor substrates.