Browsing by Author "Lu, Yen-Pei"

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A Miniature CMOS Optoelectronic Biosensor for Screening Skin-Whitening Agents
(2009)
Chang, Yu-Wei
;
Lu, Yen-Pei
;
Lin, Ming-Yu
;
Huang, Yang-Tung
;
Yang, Yuh-Shyong
A miniature CMOS optoelectronic biosensor is proposed for screening whitening agents. Based on the principle of absorption photometry, this biosensor is mainly composed of a green LED, a CMOS phototransistor, and a slide for performing the biochemical reactions. The requirement of the sample volume is only 10 mu l, much less than those in commercial instruments. Melanin assay and MTT assay were performed to evaluate the whitening mechanisms. Experimental results demonstrated that the density of 5x10(4) cells/ml can be successfully detected for both assays. This portable biosensor has great potential toward personalized diagnostic kits for further applications.
Alignment of Stretchable Nanoparticle Chains with Tunable Optical Properties Formed from Molecular Machinery
(2013-01-01)
Lin, Ming-Yu
;
Lu, Yen-Pei
;
Yang, Yuh-Shyong
;
Chen, Hsuen-Li
;
Yang, Chih-Hui
Control orientation and interparticle spacing of self-assembled nanoparticles can provide versatile functional materials for optical and electronic applications. In this study, we have obtained a glass substratum with tunable optical properties using flowing control and bimolecular competition. The surface of glass was modified from immobilized stretchable gold nanoparticle chains. The long and periodic single-stranded DNA nanotemplates were prepared using Phi29 DNA polymerase molecular machinery according to precise molecular biology regulation. Gold nanoparticle (Au NP) docked at the default sites of the nanotemplates and optical anisotropic absorption were enhanced by approximately 55 % by using tunable, flexible, and oriented one-dimensional metallic nanostructures.
Tumor Marker Detection by Aptamer-Functionalized Graphene Oxide
(2013-01-01)
Lin, Ming-Yu
;
Lu, Yen-Pei
;
Grumezescu, Alexandru Mihai
;
Ho, Fu Han
;
Kao, Ying-Hsien
Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors (PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions on GO-based biosensors, bioreactors, and other devices.