Browsing by Author "Osaki, Dai"

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Citrullination and deamidation affect aggregation properties of amyloid -proteins
(2016-12)
Osaki, Dai
;
Hiramatsu, Hirotsugu
;
應用化學系
;
Department of Applied Chemistry
Citrullination and deamidation, which are aging-related posttranslational modifications, increase the number of negative charges on amyloid -protein (A) at neutral pH. We investigated the effects of these modifications on the fibrillation properties of A. The Arg5Cit modification of A(1-40) did not affect the fibrillation rate, and brought -sheet structures unlike that in the A(1-40) fibril. The Asn27Asp modification of A(1-40) stopped the fibrillation and induced the formation of aggregates that involved an anti-parallel -sheet. A(1-42) with the Arg5Cit modification showed increased solubility in aqueous media, and its fibril formation became slower than that of A(1-42). The modification did not change the parallel -sheet structure of the fibrils. A(1-42) with the Asn27Asp modification partially formed fibrils that involved the parallel -sheet structure. Using the thioflavin T (ThT) assay, an increased fraction of the soluble oligomer of each A analog was transiently detected during fibrillation. An increase in the number of negative charges at basic pH affected the aggregation properties of A in a manner different from that with the modifications, suggesting that change in properties of the posttanslationally modified residues rather than the number of charges in the peptide was important.