Browsing by Author "Peng, Kou-Cheng"

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Different effects of probiotic species/strains on infections in preschool children: A double-blind, randomized, controlled study
(2009-02-11)
Lin, Jun-Song
;
Chiu, Yi-Han
;
Lin, Nien-Tsung
;
Chu, Chia-Hsiang
;
Huang, Kuo-Chin
Treatment and prevention of pediatric infectious diseases of three commercial probiotic products were evaluated by a double-blind, randomized, controlled trial. Test subjects under age 5,1062 in total, were distributed randomly into four groups. This investigation showed that L. casei rhamnosus can control bacterial, viral and respiratory infections; a multi-species probiotic reduced gastrointestinal disease significantly. Long-term consumption of L. rhamnosus T cell-1 decreased the incidence of bacterial infection. (C) 2008 Elsevier Ltd. All rights reserved.
Preferential promotion of apoptosis of monocytes by Lactobacillus casei rhamnosus soluble factors
(2010-02-01)
Chiu, Yi-Han
;
Hsieh, Yi-Jen
;
Liao, Kuang-Wen
;
Peng, Kou-Cheng
;
生物科技學系
Background & aims: inflammatory bowel disease (IBD) is characterized by dense infiltrates of and defective apoptosis by mucosal cell populations. Some probiotics inhibit monocytes' expansion, although mechanisms remain unknown. Supernatants of Lactobacillus strains were investigated for inducing apoptosis of monocytes. Methods: Secreted factors produced by Latobacillus strains were tested on human lymphocytes, monocytes and a human monocytic leukemia-cell line (THP-1). Cell death mechanisms were investigated by a variety of methods. Lipopolysaccharide (LPS)-induced proinflammatory cytokines (IL-1 beta, IL-6, IL-8, TNF-alpha) and anti-inflammatory TGF-beta 1 were determined. Results: Soluble factor(s) from Lactobacillus casei rhamnosus strain supernatants (LcrS) effectively induced apoptosis of immune cells. These were mainly soluble proteins (MW 5-30 kDa; LcrS(5-30)). For immune cells, but not human colonic epithelial carcinoma cells (HT-29), pretreatment with LcrS(5-30) significantly promoted apoptosis via a mitochondrial pathway. LcrS(5-30) suppressed pro-inflammatory cytokines and induced anti-inflammatory TGF-beta 1. Conclusions: Probiotic Lcr produced heat-stable molecules (MW range 5-30 kDa) that promoted immune cell apoptosis without affecting intestinal epithelial cells. LcrS(5-30) triggered apoptosis by a mitochondrial pathway, but not via TGF-beta signaling pathway. LcrS(5-30) also inhibited LPS-induced inflammatory cytokines by immune cells. Thus, LcrS(5-30) promotes apoptosis of immune cells, and suggests probiotics-based regimens for prevention of IBD. (C) 2009 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.