Browsing by Author "Peng, Sheng-Lung"

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A Novel Method for Designing Information Technology Services
(2016-01-01)
Kao, Yi-Chih
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Chang, Yung-Chia
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Peng, Sheng-Lung
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Chang, Ruay-Shiung
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交大名義發表
Information technology (IT) services are diverse and complex. Numerous service design methods have been developed for designing and developing products. However, owing to the limited availability of useful tools in IT service design, these methods are ineffective. This study proposes an innovative three-dimensional method for designing IT services. The proposed method considers user requirements, an organization's business requirements, service providers' management requirements as well as the decision-making criteria of management representatives to ensure a smooth implementation of a designed IT service. Using this method, a prototype system to improve campus wireless local area network services was developed and tested at a university in Taiwan. The prototype system reduced the need for repetitive authentication and the time required to solve service problems and address user complaints. In addition, the service design team observed an approximately 70% reduction in project cycle time. The proposed method provided a systematic means to organize the design and implementation of IT services.
CAPS Genomic Subtyping on Orthomyxoviridae
(2009)
Peng, Sheng-Lung
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Tsay, Yu-Wei
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Lin, Chich-Sheng
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Tang, Chuan Yi
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生物科技學系
The Orthomyxoviridae is a family of single strained RNA viruses including five genera: Influenza virus A, Influenza virus B, Influenza virus C, Thogotovirus, and Isavirus. Usually, Influenza viruses are identified by antigenic differences in their nucleoprotein and matrix protein. In this paper, we propose an algorithm to determine a set of suitable: restriction enzymes for producing recognizable restriction maps on Orthomyxoviridae. Our method is applied to viral strains of highly pathogenic avian influenza (HPAI), containing potentially homozygous, heterozygous; and various genetic variations. In the analysis of CAPS (Cleaved Amplified Polymorphic Sequence) subtyping, our method out; performs the RNA coding of representative and epidemiologically significant human wild-type viruses, including H3N8, H5N1, H5N9, H7N1, H7N7, and H9N2. These isolates are analyzed by CAPS with enzymes Agel, EciI, KpnI, and XbaI. The HPAI strains show a. different RFLP (Restriction Fragment Length Polymorphism) profile by comparing with other low pathogenic avian influenza (LPAI) strains. We provide a rapid; specific, and reproducible identification of the genotypes on Orthomyxoviridae It permits us to quickly confirm subtypes of Orthomyxoviridae.
Optical Detection of Human Papillomavirus Type 16 and Type 18 by Sequence Sandwich Hybridization With Oligonucleotide-Functionalized Au Nanoparticles
(2009-06-01)
Chen, Sz-Hau
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Lin, Kun-I
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Tang, Chuan-Yi
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Peng, Sheng-Lung
;
Chuang, Yao-Chen
The importance of detecting and subtyping human papillomaviruses (HPVs) in clinical and epidemiological studies has been well addressed. In detecting the most common types of HPV, type 16 (HPV-16) and type 18 (HPV-18), in the cervical mucous of patients in a simple and rapid manner, the assay of a label-free colorimetric DNA sensing method based on sequence sandwich hybridization with oligonucleotide-functionalized Au nanoparticles (AuNPs) was fabricated in this study. Specific oligonucleotide probes were designed for the sequence detection within the L1 gene of HPV-16 and HPV-18, and the probes were capped onto AuNPs, as AuNP probes. The target HPV sequences in clinical specimens were obtained by an asymmetric polymerase chain reaction (PCR) with universal primers, which can amplify the target sequences from several HPV serotypes, including HPV-16 and HPV-18. The DNA sandwich hybridization between the target sequences and the specific AuNP probes was performed at a temperature closer to the theoretical melting temperature of the DNA hybridization. Next, the procedure of increasing salt concentration and cooling the hybridizing solution was immediately utilized to discriminate the target sequences of HPV-16 or HPV-18. If the target sequences were not complementary to sequences of AuNP probes, the AuNPs would aggregate because no duplex DNA formation occurred such that the color of the reaction solution changed from red to purple. If the AuNP probes were a perfect match to the target sequences and a full DNA sandwich hybridization occurred, the reaction solution maintained its red color. A total of 70 mucous specimens from patients with cervical intraepithelial neoplasia were tested by the AuNP probes sandwich hybridization. The results show that there were 33, 16, 5, and 16 cases detected with HPV-16, HPV-18, both HPV-16 and HPV-18 (HPV-16/HPV-18), and neither HPV-16 nor HPV-18, respectively. In comparison with the specific detection by TaqMan real-time PCR assays for HPV-16, the detection sensitivity and specificity of the AuNP probes sandwich hybridization reached 95% and 90%, respectively, for HPV-16 diagnosis.