Browsing by Author "Peng, Shu-Chun"

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A novel role of CPEB3 in regulating EGFR gene transcription via association with Stat5b in neurons
(2010-11-01)
Peng, Shu-Chun
;
Lai, Yen-Ting
;
Huang, Hsi-Yuan
;
Huang, Hsien-Da
;
Huang, Yu-Shuian
CPEB3 is a sequence-specific RNA-binding protein and represses translation of its target mRNAs in neurons. Here, we have identified a novel function of CPEB3 as to interact with Stat5b and inhibit its transcription activity in the nucleus without disrupting dimerization, DNA binding and nuclear localization of Stat5b. Moreover, CPEB3 is a nucleocytoplasm-shuttling protein with predominant residence in the cytoplasm; whereas activation of NMDA receptors accumulates CPEB3 in the nucleus. Using the knockdown approach, we have found the receptor tyrosine kinase, EGFR, is a target gene transcriptionally activated by Stat5b and downregulated by CPEB3 in neurons. The increased EGFR expression in CPEB3 knockdown neurons, when stimulated with EGF, alters the kinetics of downstream signaling. Taken together, CPEB3 has a novel function in the nucleus as to suppress Stat5b-dependent EGFR gene transcription. Consequently, EGFR signaling is negatively regulated by CPEB3 in neurons.
A novel role of CPEB3 in regulating EGFR gene transcription via association with Stat5b in neurons
(2010-11-01)
Peng, Shu-Chun
;
Lai, Yen-Ting
;
Huang, Hsi-Yuan
;
Huang, Hsien-Da
;
Huang, Yu-Shuian
CPEB3 is a sequence-specific RNA-binding protein and represses translation of its target mRNAs in neurons. Here, we have identified a novel function of CPEB3 as to interact with Stat5b and inhibit its transcription activity in the nucleus without disrupting dimerization, DNA binding and nuclear localization of Stat5b. Moreover, CPEB3 is a nucleocytoplasm-shuttling protein with predominant residence in the cytoplasm; whereas activation of NMDA receptors accumulates CPEB3 in the nucleus. Using the knockdown approach, we have found the receptor tyrosine kinase, EGFR, is a target gene transcriptionally activated by Stat5b and downregulated by CPEB3 in neurons. The increased EGFR expression in CPEB3 knockdown neurons, when stimulated with EGF, alters the kinetics of downstream signaling. Taken together, CPEB3 has a novel function in the nucleus as to suppress Stat5b-dependent EGFR gene transcription. Consequently, EGFR signaling is negatively regulated by CPEB3 in neurons.
THE POTENTIAL MARKET OF AIRPORT RAIL RAPID TRANSIT
(2006)
Hsu, Chaug-Ing
;
Chang, Fa-Chu
;
Peng, Shu-Chun
;
運輸與物流管理系註：原交通所+運管所
;
Department of Transportation and Logistics Management
With the increasing ground access demand in and out of airport, there is more and more international airport Rail Rapid Transit (RRT) around the world. However, previous research rarely considered the effect of weight of baggage on traveler's choice on access mode. This study presents an analytical model to analyze individual choices and market demands by travelers, who travel to the airport with two choices, i.e. using airport RRT or driving a car via freeway. Finally, the study presents an example to demonstrate the application of the model and to analyze the market shares of two modes used by travelers from Taipei City to Chiang Kai Shek (CKS) international airport in Taiwan. The results show that the market shares of airport RRT will be markedly larger at peak periods than those at off-peak periods, and with an average of 60 percent during a day.