Browsing by Author "Perevedentseva, E."

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Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling
(2007-04-16)
Cheng, C.-Y.
;
Perevedentseva, E.
;
Tu, J.-S.
;
Chung, P.-H.
;
Cheng, C.-L.
This letter presents direct observation of growth hormone receptor in one single cancer cell using nanodiamond-growth hormone complex as a specific probe. The interaction of surface growth hormone receptor of A549 human lung epithelial cells with growth hormone was observed using nanodiamond's unique spectroscopic signal via confocal Raman mapping. The growth hormone molecules were covalent conjugated to 100 nm diameter carboxylated nanodiamonds, which can be recognized specifically by the growth hormone receptors of A549 cell. The Raman spectroscopic signal of diamond provides direct and in vitro observation of growth hormone receptors in physiology condition in a single cell level. (C) 2007 American Institute of Physics.
Spectroscopic study of bio-functionalized nanodiamonds
(2006-04-01)
Chung, P. -H.
;
Perevedentseva, E.
;
Tu, J. -S.
;
Chang, C. C.
;
Cheng, C. -L.
Nano-scale materials with attached biomolecules on the surface can be used as bioprobes in cell and tissue analysis. The conjugation of these nanoparticles with biomolecules is one of the key steps in the development of bioprobes. In this work nanometer-sized diamonds (5 and 100 nm) are functionalized and conjugated with protein lysozyme via physical adsorption. The process of creating functional groups on the nanodiamond surface is studied using Fourier Transform Infrared-spectroscopy (FTIR). The conjugation of nanodiamonds with biontolecules and nanodiamond-biomolecule interaction are analyzed with UV/VIS and FTIR spectroscopy. (c) 2005 Elsevier B.V. All rights reserved.