Browsing by Author "Primadona, Indah"

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Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions
(2014)
Chien, Wei-Ting
;
Liang, Chien-Fu
;
Yu, Ching-Ching
;
Lin, Chien-Hung
;
Li, Si-Peng
A simple and efficient protocol for the preparative-scale synthesis of various lengths of oligo-N-acetyllactosamine (oligo-LacNAc) and its multi-sialylated extensions is described. The strategy utilizes one thermophilic bacterial thymidylyltransferase (RmlA) coupled with corresponding sugar-1-phosphate kinases to generate two uridine diphosphate sugars, UDP-galactose and UDP-N-acetylglucosamine. By incorporating glycosyltransferases, oligo-LacNAcs and their sialylated analogs were synthesized.