Browsing by Author "Segawa, Hiroki"

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Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation)
(2012-04-23)
Segawa, Hiroki
;
Okuno, Masanari
;
Kano, Hideaki
;
Leproux, Philippe
;
Couderc, Vincent
We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), THG (third harmonic generation) and multiplex TSFG (third-order sum frequency generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall. (C) 2012 Optical Society of America
Time-frequency resolved analysis of a nanosecond supercontinuum source dedicated to multiplex CARS application
(2012-12-31)
De Angelis, Annalisa
;
Labruyere, Alexis
;
Couderc, Vincent
;
Leproux, Philippe
;
Tonello, Alessandro
In this paper, we describe and investigate the properties of a broadband source designed from a nanosecond microchip laser operating at high repetition rate and dedicated to multiplex-CARS application. We demonstrate that a strong reshaping of the initial pulse profile drastically affects the Stokes wave and therefore represents an important limitation in CARS experiment. In particular, we emphasize the saturation effect of the peak power of the Stokes wave resulting from supercontinuum generation. However, we show that this type of compact system can be particularly suitable for achieving CARS measurement. (C) 2012 Optical Society of America