Browsing by Author "Sun, Chia-Wei"

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Altered near-infrared spectroscopy response to breath-holding in patients with fibromyalgia
(2019-01-01)
Chen, Wei-Ta
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Yu, Chuan-Hsiang
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Sun, Chia-Wei
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交大名義發表
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National Chiao Tung University
Fibromyalgia (FM) is a complex syndrome characterized by chronic widespread pain and a heightened response to pressure. Most medical researches pointed out that FM patients with endothelial dysfunction and arterial stiffness. A continuous-wave near-infrared spectroscopy (NIRS) system is used in present study to measure the hemodynamic changes elicited by breath-holding task in patients with FM. Each patient completed a questionnaire survey including demographics, characteristics of body pain, associated symptoms, headache profiles and Hospital Anxiety and Depression Scale. A total of 27 FM patients and 26 health controls were enrolled. In comparison with healthy controls, patients with FM showed lower maximal and averaged change of oxyhemoglobin concentration in both the left (1.634 +/- 0.890 and 0.810 +/- 0.525 mu M) and the right (1.576 +/- 0.897 and 0.811 +/- 0.601 mu M) prefrontal cortex than healthy controls (P < .05 for both sides) during the breath-holding task. In conclusion, FM is associated with altered cerebrovascular reactivity measured by NIRS and breath-holding task, which may reflect endothelial dysfunction or arterial stiffness. Oxygenated hemoglobin concentration changes of healthy controls and FM patients.
Brain structure and spatial sensitivity profile assessing by near-infrared spectroscopy modeling based on 3D MRI data
(2013-03-01)
Chuang, Ching-Cheng
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Chen, Chung-Ming
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Hsieh, Yao-Sheng
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Liu, Tsan-Chi
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Sun, Chia-Wei
The goal of this study is to prove that the light propagation in the head by used the 3-D optical model from in vivo MRI data set can also provide significant characteristics on the spatial sensitivity of cerebral cortex folding geometry based on Monte Carlo simulation. Thus, we proposed a MRI based approach for 3-D brain modeling of near-infrared spectroscopy (NIRS). In the results, the spatial sensitivity profile of the cerebral cortex folding geometry and the arrangement of source-detector separation have being necessarily considered for applications of functional NIRS. The optimal choice of source-detector separation is suggested within 33.5 cm by the received intensity with different source-detector separations and the ratio of received light from the gray and white matter layer is greater than 50%. Additionally, this study has demonstrated the capability of NIRS in not only assessing the functional but also detecting the structural change of the brain by taking advantage of the low scattering and absorption coefficients observed in CSF of sagittal view. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Characterization of tooth structure and the dentin-enamel zone based on the Stokes-Mueller calculation
(2012-11-01)
Sun, Chia-Wei
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Hsieh, Yao-Sheng
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Ho, Yi-Ching
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Jiang, Cho-Pei
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Chuang, Ching-Cheng
This is the first study of dentin-enamel zone (DEZ) identification with tooth structure characterization based on the optical Stokes-Mueller measurement. Stokes vectors of a cross-sectional tooth slice were measured using various polarization inputs. The direction of the DEZ is different in enamel and dentin structures; therefore, the Stokes profiles can specifically characterize the structures based on the DEZ. This optical method, using polarimetry, provides a useful tool for characterizing tooth. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.11.116026]
Combination of Electroencephalography and Near-Infrared Spectroscopy in Evaluation of Mental Concentration during the Mental Focus Task for Wisconsin Card Sorting Test
(2017-03-23)
Chen, Che-Wei
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Sun, Chia-Wei
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光電工程學系
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Department of Photonics
Near-infrared spectroscopy (NIRS) is a noninvasive neuroimaging tool for measuring evoked functional changes in brain oxygenation. Electroencephalography (EEG) can be used to evaluate the functionality of cortical connections and obtain information on regional cortical activity. Coregistration of EEG-NIRS is a recent technique that has been applied for measuring changes in electrical and hemodynamic activity in the human brain. EEG-NIRS coregistration facilitates the avoidance of misleading interpretations of NIRS, particularly in the diagnosis of neurological disorders. In this study, we investigated an approach for enhancing accuracy of NIRS by using EEG to monitor physiological activity during a mental focus task. Using the Wisconsin Card Sorting Test for the subjects mental focus task, we identified two trend types in the EEG and NIRS signals of normal subjects. These data can assist in understanding brain activation statuses and enable determining subjects' degree of mental concentration. If the data can be standardized for the diagnosis of neurological disorders, they can provide a new index to improve traditional methods (e.g., questionnaires) to assist clinical doctors in diagnosing cognitive disorders.
Dental Calculus Image Based on Optical Coherence Tomography
(2011)
Hsieh, Yao-Sheng
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Ho, Yi-Ching
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Lee, Shyh-Yuan
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Chuang, Ching-Cheng
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Wang, Chun-Yang
In this study, the dental calculus was characterized and imaged by means of swept-source optical coherence tomography (SSOCT). The refractive indices of enamel, dentin, cementum and calculus were measured as 1.625+/-0.024, 1.534+/-0.029, 1.570+/-0.021 and 1.896+/-0.085, respectively. The dental calculus lead strong scattering property and thus the region can be identified under enamel with SSOCT imaging. An extracted human tooth with calculus was covered by gingiva tissue as in vitro sample for SSOCT imaging.
Dental Optical Coherence Tomography
(2013-07-01)
Hsieh, Yao-Sheng
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Ho, Yi-Ching
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Lee, Shyh-Yuan
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Chuang, Ching-Cheng
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Tsai, Jui-che
This review paper describes the applications of dental optical coherence tomography (OCT) in oral tissue images, caries, periodontal disease and oral cancer. The background of OCT, including basic theory, system setup, light sources, spatial resolution and system limitations, is provided. The comparisons between OCT and other clinical oral diagnostic methods are also discussed.
Diffuse Optical Multipatch Technique for Tissue Oxygenation Monitoring: Clinical Study in Intensive Care Unit
(2012-01-01)
Wang, Chun-Yang
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Chuang, Ming-Lung
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Liang, Shinn-Jye
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Tsai, Jui-che
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Chuang, Ching-Cheng
Diffuse optical multipatch technique is used to assess spatial variations in absorption and scattering in biological tissue, by monitoring changes in the concentration of oxyhemoglobin and deoxyhemoglobin. In our preliminary study, the temporal tracings of tissue oxygenation are measured using diffuse optical multipatch measurement and a venous occlusion test, employing normal subjects and ICU patients suffering from sepsis and heart failure. In experiments, obvious differences in tissue oxygenation signals were observed among all three groups. This paper discusses the physiological relevance of tissue oxygenation with respect to disease.
Diffuser-Aided Diffuse Optical Imaging for Breast Tumor: A Feasibility Study Based on Time-Resolved Three-Dimensional Monte Carlo Modeling
(2012-05-01)
Chuang, Ching-Cheng
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Lee, Chia-Yen
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Chen, Chung-Ming
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Hsieh, Yao-Sheng
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Liu, Tsan-Chi
This study proposed diffuser-aided diffuse optical imaging (DADOI) as a new approach to improve the performance of the conventional diffuse optical tomography (DOT) approach for breast imaging. The 3-D breast model for Monte Carlo simulation is remodeled from clinical MRI image. The modified Beer-Lambert's law is adopted with the DADOI approach to substitute the complex algorithms of inverse problem for mapping of spatial distribution, and the depth information is obtained based on the time-of-flight estimation. The simulation results demonstrate that the time-resolved Monte Carlo method can be capable of performing source-detector separations analysis. The dynamics of photon migration with various source-detector separations are analyzed for the characterization of breast tissue and estimation of optode arrangement. The source-detector separations should be less than 4 cm for breast imaging in DOT system. Meanwhile, the feasibility of DADOI was manifested in this study. In the results, DADOI approach can provide better imaging contrast and faster imaging than conventional DOT measurement. The DADOI approach possesses great potential to detect the breast tumor in early stage and chemotherapy monitoring that implies a good feasibility for clinical application.
Diffuser-aided time-domain diffuse optical imaging: a phantom study
(2014-04-01)
Sun, Chia-Wei
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Chuang, Ching-Cheng
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Lee, Chia-Yen
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Chen, Chung-Ming
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光電工程學系
We present the first experimental results of time-resolved diffuser-aided diffuse optical imaging (DADOI) method in this paper. A self-manufactured diffuser plate was inserted between the optode and the surface of a scattering medium. The diffuser was utilized to enhance the multiple scattering that destroys the image information for baseline measurement of turbid medium. Therefore, the abnormality can be detected with the modified optical density calculation. The time-domain DADOI method can provide better imaging contrast and simpler imaging than the conventional diffuse optical tomography measurement. Besides, it also reveals rich depth information with temporal responses. Therefore, the DADOI offers a great potential to detect the breast tumor and chemotherapy monitoring in clinical diagnosis. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Discriminant analysis of functional optical topography for schizophrenia diagnosis
(2014-01-01)
Chuang, Ching-Cheng
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Nakagome, Kazuyuki
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Pu, Shenghong
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Lan, Tsuo-Hung
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Lee, Chia-Yen
Abnormal prefrontal function plays a central role in the cognition deficits of schizophrenic patients; however, the character of the relationship between discriminant analysis and prefrontal activation remains undetermined. Recently, evidence of low prefrontal cortex (PFC) activation in individuals with schizophrenia has also been found during verbal fluency tests (VFT) and other cognitive tests with several neuroimaging methods. The purpose of this study is to assess the hemodynamic changes of the PFC and discriminant analysis between schizophrenia patients and healthy controls during VFT task by utilizing functional optical topography. A total of 99 subjects including 53 schizophrenic patients and 46 age- and gender-matched healthy controls were studied. The results showed that the healthy group had larger activation in the right and left PFC than in the middle PFC. Besides, the schizophrenic group showed weaker task performance and lower activation in the whole PFC than the healthy group. The result of the discriminant analysis showed a significant difference with P value <0.001 in six channels (CH 23, 29, 31, 40, 42, 52) between the schizophrenic and healthy groups. Finally, 68.69% and 71.72% of subjects are correctly classified as being schizophrenic or healthy with all 52 channels and six significantly different channels, respectively. Our findings suggest that the left PFC can be a feature region for discriminant analysis of schizophrenic diagnosis. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Functional Connectivity During Phonemic and Semantic Verbal Fluency Test: A Multichannel Near Infrared Spectroscopy Study
(2016-05-06)
Huang, Chun-Jung
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Chou, Po-Han
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Wei, Hao-Lin
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Sun, Chia-Wei
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光電工程學系
Verbal fluency tests (VFTs) are widely used frontal lobe neuropsychological tests. They have been frequently used in various functional brain mapping studies. There are two versions of VFTs based on the type of cue: the letter fluency task (LFT) and the category fluency task (CFT). However, the fundamental aspects of brain connectivity across the frontotemporal regions during the VFTs have not been elucidated. In this study, we hypothesized that differences in cortical functional connectivity over the left and right frontotemporal regions may be observed by means of multi-channel functional near-infrared spectroscopy (fNIRS) during the performance of LFT and CFT. Our results from fNIRS (ETG-4000) showed different patterns of brain functional connectivity during the two types of VFTs, which was consistent with the different cognitive requirements of each task. We demonstrate increased brain functional connectivity over the frontal and temporal regions during the LFT than during the CFT; these results are in line with previous brain activity studies using fNIRS to demonstrate higher frontal and temporal region activation during LFT and CFT, with more pronounced frontal activation by the LFT.
"Functional Connectivity During Phonemic and Semantic Verbal Fluency Test: A Multichannel Near Infrared Spectroscopy Study" (vol 22, pg 43, 2016)
(2019-11-01)
Huang, Chun-Jung
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Chou, Po-Han
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Lin, Wei-Hao
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Sun, Chia-Wei
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交大名義發表
Gender-Related Effect in Oxygenation Dynamics by Using Far-Infrared Intervention with Near-Infrared Spectroscopy Measurement: A Gender Differences Controlled Trial
(2015-11-10)
Kao, Wei-Lung
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Sun, Chia-Wei
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光電工程學系
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Department of Photonics
Many studies have indicated the microcirculation can directly respond to disease-related symptoms. However, the capacity of microcirculation would vary due to the gender differences. Near-infrared spectroscopy (NIRS) is a noninvasive technique to monitor tissue oxygenation dynamics. In this study, the far-infrared (FIR) source was used for physiological intervention of microcirculation. The experimental results show that the nature difference of oxygenation status exists between male and female during FIR irradiation. Therefore, we suggest the NIRS-based assessment should be calibrated with the gender-related effect for clinical diagnosis of peripheral arterial disease.
Gender-related effects of prefrontal cortex connectivity: a resting-state functional optical tomography study
(2014-08-01)
Chuang, Ching-Cheng
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Sun, Chia-Wei
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交大名義發表
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分子醫學與生物工程研究所
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光電工程學系
The prefrontal cortex (PFC) is thought to play an important role in "higher" brain functions such as personality and emotion that may associated with several gender-related mental disorders. In this study, the gender effects of functional connectivity, cortical lateralization and significantly differences in the PFC were investigated by using resting-state functional optical tomography (fOT) measurement. A total of forty subjects including twenty healthy male and twenty healthy female adults were recruited for this study. In the results, the hemoglobin responses are higher in the male group. Additionally, male group exhibited the stronger connectivity in the PFC regions. In the result of lateralization, leftward dominant was observed in the male group but bilateral dominance in the female group. Finally, the 11 channels of the inferior PFC regions (corresponding to the region of Brodmann area 45) are significant different with spectrum analysis. Our findings suggest that the resting-state fOT method can provide high potential to apply to clinical neuroscience for several gender-related mental disorders diagnosis. (C)2014 Optical Society of America
GHz Optical Time-Stretch Microscopy by Compressive Sensing
(2017-04-01)
Lei, Cheng
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Wu, Yi
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Sankaranarayanan, Aswin C.
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Chang, Shih-Min
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Guo, Baoshan
Optical time-stretch microscopy has recently attracted intensive attention for its capability of acquiring images at an ultrahigh frame rate. Unfortunately, its achievable frame rate is limited by the requirement of having no overlap between consecutive frames, which leads to a tradeoff between the frame rate (pulse repetition rate) and the amount of the temporal dispersion used for optical image serialization. In this paper, we demonstrate compressive sensing on the platform of optical time-stretch microscopy to overcome the tradeoff between frame rate and temporal dispersion (time stretch) and achieve 50 times higher frame rate than conventional optical time-stretch microscopy. Specifically, we computationally perform compressed optical time-stretch microscopy with an experimental dataset acquired by conventional optical time-stretch microscopy and demonstrate its effects in terms of spatial resolution and cell classification accuracy. Our results indicate that the spatial resolution and cell classification accuracy reach 780 nm and 95% at a line scan rate of 675 MHz and 6.75 GHz, respectively, which correspond to five times and 50 times higher frame rates than what conventional optical time-stretch microscopy can achieve with the same dispersion amount and digitizer sampling rate.
Hemodynamic analysis of patients in intensive care unit based on diffuse optical spectroscopic imaging system
(2010)
Hsieh, Yao-Sheng
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Wang, Chun-Yang
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Ling, Yo-Wei
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Chuang, Ming-Lung
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Chuang, Ching-Cheng
Diffuse optical spectroscopic imaging (DOSI) is a technique to assess the spatial variation in absorption and scattering properties of the biological tissues and provides the monitoring of changes in concentrations of oxy-hemoglobin and deoxy-hemoglobin. In our preliminary study, the temporal tracings of hemodynamic oxygenation are measured with DOSI and venous occlusion test (VOT) from normal subjects, patients with heart failure and patients with sepsis in intensive care unit (ICU). In experiments, the obvious differences of hemodynamic signals can be observed among the three groups. The physiological relevance of VOT hemodynamics with respect to diseases is also discussed in this paper.
High-efficiency and low assembly-dependent chip-scale package for white light-emitting diodes
(2015-04-21)
Huang, Che-Hsuan
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Chen, Kuo-Ju
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Tsai, Ming-Ta
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Shih, Min-Hsiung
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Sun, Chia-Wei
This article presents a chip-scale package (CSP) with conformal and uniform structures for white light-emitting diodes used in lighting and backlight unit (BLU) applications. The CSP structures produce higher light extraction efficiency and lower assembly-dependent packaging compared with conventional surface-mounted devices (SMDs). Simulation results show that compared with SMDs, the luminous efficiency of CSP structures is 8.81% higher in lighting applications and 9.43% higher in BLU applications. This is likely due to light loss in the light bowl of the SMDs. Moreover, CSPs with a conformal phosphor structure exhibit low assembly dependence and redundancy, and rb-CSPs with a conformal structure are a more effective light source in both lighting and BLU applications. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Intelligent classification of platelet aggregates by agonist type
(2020-05-12)
Zhou, Yuqi
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Yasumoto, Atsushi
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Lei, Cheng
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Huang, Chun-Jung
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Kobayashi, Hirofumi
Platelets are anucleate cells in blood whose principal function is to stop bleeding by forming aggregates for hemostatic reactions. In addition to their participation in physiological hemostasis, platelet aggregates are also involved in pathological thrombosis and play an important role in inflammation, atherosclerosis, and cancer metastasis. The aggregation of platelets is elicited by various agonists, but these platelet aggregates have long been considered indistinguishable and impossible to classify. Here we present an intelligent method for classifying them by agonist type. It is based on a convolutional neural network trained by high-throughput imaging flow cytometry of blood cells to identify and differentiate subtle yet appreciable morphological features of platelet aggregates activated by different types of agonists. The method is a powerful tool for studying the underlying mechanism of platelet aggregation and is expected to open a window on an entirely new class of clinical diagnostics, pharmacometrics, and therapeutics.
Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging
(2020-01-06)
Wu, Yunzhao
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Zhou, Yuqi
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Huang, Chun-Jung
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Kobayashi, Hirofumi
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Yan, Sheng
Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,0(X)cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Intelligent Image De-Blurring for Imaging Flow Cytometry
(2019-05-01)
Zhang, Fangzheng
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Lei, Cheng
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Huang, Chun-Jung
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Kobayashi, Hirofumi
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Sun, Chia-Wei
By virtue of the combined merits of optical microscopy and flow cytometry, imaging flow cytometry is a powerful tool for rapid, high-content analysis of single cells in large heterogeneous populations. However, its efficiency (defined by the ratio of the number of clearly imaged cells to the total cell population) is not high (typically 50-80%), due to out-of-focus image blurring caused by imperfect fluidic focusing of cells, a common drawback that not only reduces the number of cell images useable for high-content analysis but also increases the probability of false events and missed rare cells. To address this challenge and expand the efficacy of imaging flow cytometry, here, we propose and demonstrate intelligent deblurring of out-of-focus cell images in imaging flow cytometry. Specifically, by using our machine learning algorithms, we show an 11% increase in variance and a 95% increase in first-order gradient summation of cell images taken with an optofluidic time-stretch microscope. Without strict hardware requirements, our intelligent de-blurring method provides a promising solution to the out-of-focus blurring problem of imaging flow cytometers and holds promise for significantly improving their performance. (C) 2019 International Society for Advancement of Cytometry