Browsing by Author "Tsai, Chi-Li"

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A molecular forensic method for identifying species composition of processed marine mammal meats
(2014-03-01)
Chang, Chia-Hao
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Yao, Chiou-Ju
;
Yu, Hsin-Yi
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Liao, Yun-Chih
;
Jang-Liaw, Nian-Hong
We used universal primers designed for the cytochrome oxidase I (CO I) sequence of the order Cetacea and the family Phocidae to prove that meat fritters sold in Taiwan contained meat from two seal, six cetacean, and one pig species. The sequence information for CO I obtained in this study was limited and population genetics data for the eight sampled marine mammalian species was insufficient to deduce where these marine mammals were hunted. Regardless of the geographic origins of the marine mammal flesh, sale and consumption of marine mammals in Taiwan violates the Wildlife Conservation Act. This study provides PCR primers that could enable government testing of suspect meats to curtail the illegal trade in marine mammal products. (C) 2014 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.
Complete mitochondrial genome of the Chinese rasbora Rasbora steineri (Teleostei, Cyprinidae)
(2013-06-01)
Chang, Chia-Hao
;
Tsai, Chi-Li
;
Jang-Liaw, Nian-Hong
;
生物科技學系
;
Department of Biological Science and Technology
We describe the complete mitochondrial genome sequence of the Chinese rasbora, Rasbora steineri, which is a small cyprinid distributed in southeastern Asia. The circle genome (16,530 bp) consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. It has the typical vertebrate mitochondrial gene arrangement.
Complete mitochondrial genome of the Moltrecht's minnow, Aphyocypris moltrechti (Teleostei, Cyprinidae), in comparison with A. kikuchii
(2013-04-01)
Jang-Liaw, Nian-Hong
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Tsai, Chi-Li
;
Chang, Chia-Hao
;
Watanabe, Katsutoshi
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生物科技學系
We sequenced the complete mitochondrial genome of the Moltrecht's minnow, Aphyocypris moltrechti, which is known as an endemic species to Taiwan. The complete mitochondrial genome is 16,617 bp in size, consisting of 37 genes coding for 13 proteins, 2 rRNAs, 22 tRNAs, and 1 control region. Its gene arrangement pattern was identical with most vertebrates. We compared the mitochondrial genome of A. moltrechti with that of the Kikuchi's minnow, Aphyocypris kikuchii, which had been considered closely related to A. moltrechti within a same genus. Nucleotide sequence divergence (K2P distance) between the two whole mitochondrial genomes was 11.62%. The detailed comparison between the mitochondrial genomes of two species was done.
Complete mitogenomes of two Puntius in Taiwan: P-semifasciolatus and P-snyderi (Cypriniformes: Cyprinidae)
(2013-06-01)
Jang-Liaw, Nian-Hong
;
Chang, Chia-Hao
;
Tsai, Chi-Li
;
生物科技學系
;
Department of Biological Science and Technology
We sequenced the complete mitochondrial genome of two spotted barbs native to Taiwan: Puntius semifasciolatus and Puntius snyderi. The complete mitochondrial genomes are 16,594 and 16,578 bp in size, respectively. Both of them contain 37 genes coding for 13 proteins, 2 rRNAs, 22 tRNAs, and 1 control region. They share the same gene arrangement pattern that was identical with most vertebrates. Nucleotide sequence divergence (K2P distance) between the two whole mitochondrial genomes was 7.63%. These two spotted barbs show very close relationship based on the comparison of the characters of their mitochondrial genomes.