Browsing by Author "Tsai, Horng-Der"

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DELETERIOUS EFFECTS OF ARSENIC, BENOMYL AND CARBENDAZIM ON HUMAN ENDOMETRIAL CELL PROLIFERATION IN VITRO
(2010-12-01)
Chang, Chi-Chen
;
Hsieh, Yao-Yuan
;
Hsu, Kung-Hao
;
Tsai, Horng-Der
;
Lin, Wen-Hsin
Objective: We aimed to investigate the effects of arsenic (As), benomyl (Ben), and carbendazim (Carb) on endometrial cells. Materials and Methods: Human endometrial cells were obtained during diagnostic curettage. All cultured endometrial cells were divided into four groups: (1) 0 M (controls), (2) 10(-6)M, (3) 10(-5)M, (4) 10(-4)M for As, Ben and Carb. After 24 and 48 hours in culture, endometrial cell proliferations were assessed by diphenyltetrazolium bromide assay. The influences of different concentrations of As, Ben and Garb upon the endometrium were compared. Results: During the first 24 hours, As, Ben and Garb appeared to have insignificant influences upon endometrial growth. After 48 hours in culture, all three agents significantly inhibited endometrial growth. In As groups, cell absorption after 48 hours culture were 100% (group 1), 82.1% (group 2), 43.6% (group 3) and 35.3% (group 4). In Ben groups, cell absorption was 100% (1), 75.9% (2), 66.4% (3) and 49.6% (4). In the Carb groups, cell absorption was 100% (1), 70.4% (2), 73.0% (3) and 76.7% (4). Conclusion: The agents As, Ben and Carb appear to have inhibitory effects upon endometrial cells after 48 hours in culture. [Taiwan J Obstet Gynecol 2010;49(4):449-454]
Identification of novel peptides specifically binding to endometriosis by screening phage-displaying peptide libraries
(2009-12-01)
Chang, Chi-Chen
;
Hsieh, Yao-Yuan
;
Wang, Yu-Kuo
;
Hsu, Kung-Hao
;
Tsai, Horng-Der
Objective: To search for novel peptides and common binding motif that specifically bind to endometriosis. Design: Prospective study. Setting: Department of Biological Science and Technology in national university. Patient(s): Specimens were divided into [1] ectopic endometrium (n = 10); [2] eutopic endometrium (n = 10). Intervention(s): Peptides specifically binding to endometriosis are screened from a phage-displaying peptide library (Ph.D.-12) by using whole-cell screening technique after an adsorption elution amplification procedure. Main Outcome Measure(s): Combinatorial peptide libraries were used to identify small molecules that bind with high affinity to receptor molecules and mimic the interaction with natural ligands. Few pans of positive phage clones with significantly positive signals were identified by ELISA and analyzed by DNA sequencing. Result(s): During the biopanning processes, the recovered phage number (10(6) pfu/mL) in parts 1, 2, 3, 4, and 5 of the study were 9, 33, 82, 142, and 169. Nine phages consistently had residue Arg, whereas six clones had a consensus motif of Arg-X-Arg-X-X-X-X-Arg. The biotin-labeled peptide bound to endometriosis cells in a dose-dependent manner, yet the control peptide revealed lesser binding activity. Conclusion(s): The novel motif is associated with higher affinity of endometriosis, which might be useful in endometriosis targeting and as potential antiendometriosis therapies. We provide one potential approach for novel therapies toward endometriosis. (Fertil Steril (R) 2009;92:1850-5. (C)2009 by American Society for Reproductive Medicine.)