Browsing by Author "Tsai, Li-Yun"

Now showing 1 - 4 of 4

Development of a Growth- Hormone- Conjugated Nanodiamond Complex for Cancer Therapy
(2014-05-01)
Chu, Hsueh-Liang
;
Chen, Hung-Wei
;
Tseng, Shin-Hua
;
Hsu, Ming-Hua
;
Ho, Li-Ping
It is highly desirable to develop a therapeutic, observable nanoparticle complex for specific targeting in cancer therapy. Growth hormone (GH) and its antagonists have been explored as cancer cell-targeting molecules for both imaging and therapeutic applications. In this study, a low toxicity, biocompatible, therapeutic, and observable GH-nanoparticle complex for specifically targeting growth hormone receptor (GHR) in cancer cells was synthesized by conjugating GH with green fluorescence protein and carboxylated nanodiamond. Moreover, we have shown that this complex can be triggered by laser irradiation to create a nanoblast and induce cell death in the A549 non-small-cell lung cancer cell line via the apoptotic pathway. This laser-mediated, cancer-targeting platform can be widely used in cancer therapy.
Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy
(2013-07-19)
Li, Hsing-Yuan
;
Tseng, Shing-Hua
;
Cheng, Tsai-Mu
;
Chu, Hsueh-Liang
;
Lu, Yu-Ning
Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 mu 1 reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.
Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy (vol 24, 285102, 2013)
(2013-10-04)
Li, Hsing-Yuan
;
Tseng, Shing-Hua
;
Cheng, Tsai-Mu
;
Chu, Hsueh-Liang
;
Lu, Yu-Ning
Thermal stability and folding kinetics analysis of disordered protein, securin
(2014-03-01)
Chu, Hsueh-Liang
;
Chen, Tzu-Hsuan
;
Wu, Chang-You
;
Yang, Yao-Chen
;
Tseng, Shin-Hua
Lacking a stable tertiary structure, intrinsically disordered proteins (IDPs) possess particular functions in cell regulation, signaling, and controlling pathways. The study of their unique structural features, thermal stabilities, and folding kinetics is intriguing. In this study, an identified IDP, securin, was used as a model protein. By using a quasi-static five-step (on-path) folding process, the function of securin was restored and analyzed by isothermal titration calorimetry. Fluorescence spectroscopy and particle size analysis indicated that securin possessed a compact hydrophobic core and particle size. The glass transition of securin was characterized using differential scanning microcalorimetry. Furthermore, the folding/unfolding rates (k(obs)) of securin were undetectable, implying that the folding/unfolding rate is very fast and that the conformation of securin is sensitive to solvent environmental change. Therefore, securin may fold properly under specific physiological conditions. In summary, the thermal glass transition behavior and undetectable k(obs) of folding/unfolding reactions may be two of the indices of IDP.